High impact information on CRP

• Three possible promoters and a CRP binding site were identified by concensus criteria [1].
• A concentration of CRP similar to that detected in patients with cardiovascular risk (10 microg/ml) failed to modify the generation of superoxide anion stimulated by TNF-alpha [2].
• Western blot experiments showed that TNF-alpha decreased the expression of the eNOS protein, which was partially protected by treatment with 10 microg/ml CRP [2].
• Bovine CRP was composed of a single type of non-glycosylated subunit whilst bovine SAP contained two major types of glycosylated subunits and a minor polypeptide, the glycosylation of which was not determined [3].
• Latex agglutination test: a simple, rapid and practical method for bovine serum CRP determination [4].

Associations of CRP with chemical compounds

• 1. A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer [5].

Other interactions of CRP

• Addition of CRP to TNF-alpha-incubated BAEC enhanced the fluorescence signal of the CD32 receptors [2].

Analytical, diagnostic and therapeutic context of CRP

• Although the mixture of these two pentraxins was not resolved by gel filtration chromatography, they were separated by solid phase absorption of the CRP with immobilized rabbit anti-human CRP antibodies [3].
• Confocal microscopy experiments showed a weak expression of the CD32 receptor in control BAEC that was slightly increased by 10 microg/ml CRP [2].
• 2. Pure CRP and SAP were finally separated by DEAE-5PW HPLC [5].
• The critical roughage part (CRP) of 2 diet types was determined in a cross-over design with 6 double-muscled and 6 normally conformed Belgian Blue bulls fitted with rumen cannulae [6].

References