High impact information on Prg3

• In addition, a genomic clone of mMBP-2 was isolated and this gene was shown to be physically linked to within 100 kb of mMBP-1 on the central region of mouse chromosome 2 [1].
• Identification of a new murine eosinophil major basic protein (mMBP) gene: cloning and characterization of mMBP-2 [1].
• The EST was used to screen and isolate mMBP-2 from a bone marrow cDNA library [1].
• Progressive similarity alignment of the deduced mMBP-2 open reading frame demonstrates the apparent conservation of the "pre-pro-mature" protein structure found in the other known mammalian MBPs [1].
• A cDNA clone (MBP2) corresponding to a novel mouse myelin basic protein (MBP) mRNA has been isolated from an adult mouse bone marrow cDNA library [2].

Analytical, diagnostic and therapeutic context of Prg3

• Moreover, using mMBP-1 knockout mice and immunohistochemistry with an antisera that recognizes both mMBP-1 and -2, we demonstrate that mMBP-2 protein expression is restricted to eosinophil lineage-committed cells [1].
• Using PCR experiments we have determined that this MBP2 mRNA belongs to a new MBP mRNA family initiated upstream from exon 1b [2].

References