High impact information on RHOT1

• Paradigm lost: milton connects kinesin heavy chain to miro on mitochondria [1].
• In this issue Glater et al. demonstrate a new type of linkage through the adapter protein, milton, and the mitochondrial membrane GTPase, miro [1].
• Candidates include the conserved mitochondrial Rho (Miro) family of proteins, which contain two GTPase domains flanking a pair of calcium-binding EF-hand motifs [2].
• These proteins, which were called Miro-1 and Miro-2 (for mitochondrial Rho), have tandem GTP-binding domains separated by a linker region containing putative calcium-binding EF hand motifs [3].
• Immunolocalization experiments, in which transfected NIH3T3 and COS 7 cells were stained for ectopically expressed Miro as well as for the endogenous Miro-1 protein, showed that Miro was present in mitochondria [3].

Biological context of RHOT1

• ARHT may be involved in mitochondrial homeostasis and apoptosis [4].

Anatomical context of RHOT1

• It was reported previously that a constitutively active mutant of Miro-1 induced a clustering of the mitochondria [5].
• Tympanic membrane images were captured and digitized using a Welch-Allyn (Skaneatales Falls, NY) VDX-300 Illumination and Imaging system with S-VHS input to a MIRO DC 30 (Pinnacle Systems, Mountain View, CA) visual board in a Power PC-based computer [6].

Associations of RHOT1 with chemical compounds

• A certain amount of indium (0.01-1.70 mg/cm2) was solubilized from the surface of only one prosthetic semiprecious brand (Miro Bright) in either 1% lactic acid or 0.05% hydrochloric acid solutions [7].

References