High impact information on Fabp9

• The exon/intron boundaries are identical to those found in the mouse myelin P2 gene, but there is no resemblance in size and sequence between the corresponding introns of the PERF 15 and myelin P2 genes [1].
• Molecular cloning and structural analysis of the gene encoding PERF 15 protein present in the perinuclear theca of the rat spermatozoa [1].
• The PERF 15 gene contains four exons and three introns [1].
• Immunostaining was first detectable in mid-pachytene spermatocytes and increased in intensity as these cells progressed to elongated spermatids, suggesting that this testis lipid-binding protein has a specific role in sperm development [2].
• To determine whether PERF 15 participates in germ cell apoptosis, juvenile rat testis was examined by immunohistochemical and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) methods [3].

Biological context of Fabp9

• Expression of testicular fatty acid-binding protein PERF 15 during germ cell apoptosis [3].

Anatomical context of Fabp9

• We have cloned the PERF 15 gene, which encodes the most abundant protein of the perinuclear theca of rat spermatozoa [1].
• PERF 15 is a testicular germ cell specific fatty acid-binding protein (FABP) isolated from rat [3].
• Indirect immunofluorescent analysis of juvenile rat testis showed that there were some strongly PERF 15-positive spermatocytes [3].

Associations of Fabp9 with chemical compounds

• Exposure to methoxyacetic acid (MAA), known to induce apoptosis in spermatocytes, increased the number of strongly PERF 15-positive cells in 25-day-old rats' testes [3].

Analytical, diagnostic and therapeutic context of Fabp9

• Northern blot analysis demonstrated that this gene transcribed PERF 15 mRNA in meiotic and postmeiotic cells [1].

References