High impact information on BRPF1

• The latter can be arranged in three zinc fingers and shows homology to a domain within the protein Br140 (peregrin) [1].
• These structures have been found to be conserved in sequence and position in three other proteins, AF17, BR140, and a previously unrecognized Caenorhabditis elegans gene, provisionally named CEZF [2].
• To assess the potential involvement of BR140 in chromosome translocations in leukaemia, the chromosomal location of the BR140 gene has been determined by using several independent methods [3].
• The BR140 gene encodes a 140 kDa protein of unknown function that contains a putative zinc finger domain, a leucine zipper region, and, in addition, a bromo domain [3].
• The Br140 protein was initially identified due to its co-purification with an integrin [4].

Biological context of BRPF1

• A combination of Southern analysis, polymerase chain reactions (PCR) on monochromosomal cell hybrids, and fluorescence in situ hybridisation (FISH) has been used to show that the BR140 gene maps to chromosome band 3p25 [3].

Analytical, diagnostic and therapeutic context of BRPF1

• At the present time, we are engaged in the introduction of further methods, originally destined for clinical praxis, i.e. electrooculography (for laboratory purposes worked out by Manák) and electroretinography (worked out by Peregrín and Svĕrák) [5].

References