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Gene Review:

IAA7 - auxin-responsive protein IAA7

Arabidopsis thaliana

Synonyms: AUXIN RESISTANT 2, AXR2, indole-3-acetic acid 7

Cluis, C.P. et al., Leymarie, J. et al., Timpte, C. et al., Boerjan, W. et al., Nagpal, P. et al., et al.

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High impact information on IAA7

We demonstrate that SCF(TIR1) interacts with AXR2/IAA7 and AXR3/IAA17, and that domain II of these proteins is necessary and sufficient for this interaction [1].
Analysis by gas chromatography coupled to mass spectrometry indicated increased levels of both free and conjugated indole-3-acetic acid. sur1 was crossed to the mutant axr2 and the altered-auxin response mutant ctr1 [2].
Increased AXR2 expression in a hy5 background partially rescues the elongated hypocotyl phenotype [3].
The promoters of these genes contain a putative HY5 binding site, and in line with this observation, HY5 can bind to the promoter of AXR2 in vitro [3].
Auxin-induction of two late genes, IAA7 and IAA8, is inhibited by cycloheximide, indicating requirement of protein synthesis for their activation [4].

Biological context of IAA7

Linkage analysis indicates that the axr2 gene lies on chromosome 3 [5].
To learn more about the nature of the axr2-1 mutation, the effects of varying the ratio of wild-type to mutant copies of the AXR2 gene were examined by comparing plants of the following genotypes: +/+, +/+/+, axr2-1/axr2-1, axr2-1/+ and axr2-1/+/+ [6].

Associations of IAA7 with chemical compounds

We have cloned the AXR2 gene using a map-based approach, and find that it is the same as IAA7, a member of the IAA (indole-3-acetic acid) family of auxin-inducible genes [7].

Analytical, diagnostic and therapeutic context of IAA7

Microarray analyses and semiquantitative RT-PCR indicate that two negative regulators of auxin signaling, AUXIN RESISTANT 2 (AXR2)/INDOLE ACETIC ACID 7 (IAA7) and SOLITARY ROOT (SLR)/IAA14, are underexpressed in hy5 mutants [3].
In order to detect gene products involved in Arabidopsis drought adaptive strategy, 2D-PAGE protein patterns of two auxin-insensitive mutants, axr1, axr2, differentially affected in specific drought responses, were compared to the wild-type Columbia ecotype, in well-watered and drought-stressed conditions [8].

References

Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Gray, W.M., Kepinski, S., Rouse, D., Leyser, O., Estelle, M. Nature (2001) [Pubmed]
Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction. Boerjan, W., Cervera, M.T., Delarue, M., Beeckman, T., Dewitte, W., Bellini, C., Caboche, M., Van Onckelen, H., Van Montagu, M., Inzé, D. Plant Cell (1995) [Pubmed]
The Arabidopsis transcription factor HY5 integrates light and hormone signaling pathways. Cluis, C.P., Mouchel, C.F., Hardtke, C.S. Plant J. (2004) [Pubmed]
The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. Abel, S., Nguyen, M.D., Theologis, A. J. Mol. Biol. (1995) [Pubmed]
A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid. Wilson, A.K., Pickett, F.B., Turner, J.C., Estelle, M. Mol. Gen. Genet. (1990) [Pubmed]
The axr2-1 mutation of Arabidopsis thaliana is a gain-of-function mutation that disrupts an early step in auxin response. Timpte, C., Wilson, A.K., Estelle, M. Genetics (1994) [Pubmed]
AXR2 encodes a member of the Aux/IAA protein family. Nagpal, P., Walker, L.M., Young, J.C., Sonawala, A., Timpte, C., Estelle, M., Reed, J.W. Plant Physiol. (2000) [Pubmed]
Two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2, reveal interactions between drought and hormonal responses. Leymarie, J., Damerval, C., Marcotte, L., Combes, V., Vartanian, N. Plant Cell Physiol. (1996) [Pubmed]

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