High impact information on RHL2

• These results suggest that BIN3 and BIN5 may constitute an Arabidopsis topoisomerase VI that modulates expression of many genes, including those regulated by BRs [1].
• The pleiotropic dwarf phenotypes of bin5 establish that, unlike all of the other SPO11 homologs that are involved in meiosis, BIN5/AtSPO11-3 plays a major role during somatic development [1].
• In a screen designed to identify genes in the specification of epidermal cell fate in Arabidopsis primary roots we have isolated 8 new mutants that fall into 6 complementation groups corresponding to the 'root hairless' genes RHL1, RHL2 and RHL3 and the 'ectopic root hair' genes ERH1, ERH2 and ERH3 [2].
• Mutant phenotypes in the patterning and shape of leaf trichomes in rhl1, rhl2, rhl3 and erh3 were particularly obvious [2].
• A T-DNA insertion mutant of AtSPO11-3 has a phenotype that is almost to that of AtTOP6B and the double mutant [3].

Biological context of RHL2

• Thus, the three Arabidopsis Spo11 homologues appear to function in two discrete processes, i.e. AtSPO11-1 and AtSPO11-2 in meiotic recombination and AtSPO11-3 in DNA replication [4].

Other interactions of RHL2

• We isolated two extreme dwarf mutants, hypocotyl6 (hyp6) and root hairless2 (rhl2) [3], and cells of these mutants successfully complete only the first two rounds of endoreduplication and stall at 8C [5].

Analytical, diagnostic and therapeutic context of RHL2

• Furthermore, microarray analysis of the expression of about 5500 genes in bin3 or bin5 mutants indicates that about 321 genes are down-regulated in both of the mutants, including 18 of 30 BR-induced genes [1].

References