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SPO11  -  SPO11 meiotic protein covalently bound to DSB

Homo sapiens

Synonyms: CT35, Cancer/testis antigen 35, Meiotic recombination protein SPO11, SPATA43, TOPVIA
 
 
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Disease relevance of SPO11

  • Populations of infertile and fertile men were screened for mutations in SPO11 and EIF5A2, two infertility candidate genes [1].
 

High impact information on SPO11

  • Mre11 deficiency in Arabidopsis is associated with chromosomal instability in somatic cells and Spo11-dependent genome fragmentation during meiosis [2].
  • Furthermore, Spo11 expression and synaptonemal complexes, specific features of the meiotic prophase, were detected in testicular fragments cultured with DHP in some germ cells that showed morphological characteristics of undifferentiated spermatogonia [3].
  • Ski8p is cytoplasmically localized in all vegetative and sexual cycle cells, and is nuclear localized, specifically in early-mid-meiotic prophase, in temporal correlation with Spo11p, the meiotic double-strand break (DSB) transesterase [4].
  • During meiosis, recombination is initiated by the formation of DNA double strand breaks (DSBs) induced by the protein SPO11 [5].
  • The Arabidopsis MEI1 gene encodes a protein with five BRCT domains that is involved in meiosis-specific DNA repair events independent of SPO11-induced DSBs [5].
 

Biological context of SPO11

 

Anatomical context of SPO11

  • We identified a cDNA encoding Spo11 from the testis of Japanese eel, Anguilla japonica [7].
  • These results suggested that Spo11 was expressed in spermatogonia proliferated toward meiosis at quite low level that could not induce meiotic recombination, thereafter Spo11 expression increased and Dmc1 expression was initiated in early meiotic prophase [7].
  • However, immunohistochemical observations showed that Spo11 was localized only in spermatocytes [7].
 

Other interactions of SPO11

  • Screening the SPO11 and EIF5A2 genes in a population of infertile men [1].
  • TPTE, LDHC, SPO11 were expressed neither in tumor samples nor in healthy tissue [8].
 

Analytical, diagnostic and therapeutic context of SPO11

  • Western blot analysis also indicated that Spo11 production began at day 1 after hCG injection [7].
  • Spo11 transcripts were slightly detected in the testis after 1 day post-hCG injection by Northern blot analysis [7].
  • We used semi-random PCR to isolate the genomic DNA adjacent to one pPHT1 insertion in a sporulation-defective mutant and found that we had disrupted the C. cinereus spo11 gene [9].

References

  1. Screening the SPO11 and EIF5A2 genes in a population of infertile men. Christensen, G.L., Ivanov, I.P., Atkins, J.F., Mielnik, A., Schlegel, P.N., Carrell, D.T. Fertil. Steril. (2005) [Pubmed]
  2. Mre11 deficiency in Arabidopsis is associated with chromosomal instability in somatic cells and Spo11-dependent genome fragmentation during meiosis. Puizina, J., Siroky, J., Mokros, P., Schweizer, D., Riha, K. Plant Cell (2004) [Pubmed]
  3. Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel. Miura, T., Higuchi, M., Ozaki, Y., Ohta, T., Miura, C. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  4. Localization and roles of Ski8p protein in Sordaria meiosis and delineation of three mechanistically distinct steps of meiotic homolog juxtaposition. Tessé, S., Storlazzi, A., Kleckner, N., Gargano, S., Zickler, D. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  5. The Arabidopsis MEI1 gene encodes a protein with five BRCT domains that is involved in meiosis-specific DNA repair events independent of SPO11-induced DSBs. Grelon, M., Gendrot, G., Vezon, D., Pelletier, G., Mathilde, G., Ghislaine, G., Daniel, V., Georges, P. Plant J. (2003) [Pubmed]
  6. Centromere-Proximal Crossovers Are Associated With Precocious Separation of Sister Chromatids During Meiosis in Saccharomyces cerevisiae. Rockmill, B., Voelkel-Meiman, K., Roeder, G.S. Genetics (2006) [Pubmed]
  7. Molecular cloning and gene expression of Spo11 during spermatogenesis in the Japanese eel, Anguilla japonica. Ozaki, Y., Miura, C., Miura, T. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (2006) [Pubmed]
  8. Expression of cancer-testis antigens as possible targets for antigen-specific immunotherapy in head and neck squamous cell carcinoma. Atanackovic, D., Blum, I., Cao, Y., Wenzel, S., Bartels, K., Faltz, C., Hossfeld, D.K., Hegewisch-Becker, S., Bokemeyer, C., Leuwer, R. Cancer Biol. Ther. (2006) [Pubmed]
  9. Insertional mutagenesis in Coprinus cinereus: use of a dominant selectable marker to generate tagged, sporulation-defective mutants. Cummings, W.J., Celerin, M., Crodian, J., Brunick, L.K., Zolan, M.E. Curr. Genet. (1999) [Pubmed]
 
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