High impact information on WER

• Disruption of a MYB motif conserved in intron 1 of GL1, WEREWOLF, and GaMYB2 genes affected trichome production [1].
• The truncated MYB encoded by CPC mediates a lateral inhibition mechanism to negatively regulate WER, GL2, and its own gene in the alternative position (H) to induce the hair fate [2].
• To directly test the proposed role of WER in this system, we examined its subcellular localization and defined its transcriptional activation properties [3].
• In the embryonic hypocotyl we show that WER controls GL2 expression [4].
• Furthermore, an analysis of the WER and GL1 proteins shows that conserved sequences correspond to specific functional domains [5].

Biological context of WER

• Positional cues bias the expression of the WER MYB gene, leading to the induction of CPC and GL2 in cells located in a particular position (N) and adoption of the nonhair fate [2].
• Nevertheless, reciprocal complementation experiments with a series of gene fusions showed that WER and GL1 encode functionally equivalent proteins, and their unique roles in plant development are entirely due to differences in their cis-regulatory sequences [5].
• We show that WER, GL2 and CPC are expressed and active during the stages of embryogenesis when the pattern of cells in the epidermis of the root-hypocotyl axis forms [4].
• SCM regulates the expression of the GLABRA2, CAPRICE, WEREWOLF, and ENHANCER OF GLABRA3 transcription factor genes that define the cell fates [6].

Regulatory relationships of WER

• In addition, we used the glucocorticoid receptor (GR) inducible system to show that CPC transcription is regulated directly by WER [3].

Other interactions of WER

• Binding between the WER protein and both WBSs (WBSI and WBSII), and the importance of the two WBSs in CPC promoter activity were confirmed in Arabidopsis [3].

Analytical, diagnostic and therapeutic context of WER

• Furthermore, we show that these bHLH proteins require a functional WER MYB protein for their action, and they physically interact with WER and CPC in the yeast two-hybrid assay [7].

References