Disease relevance of GABPAP

• The retinoblastoma binding factor 1 (RBF-1) site in RB gene promoter binds preferentially E4TF1, a member of the Ets transcription factors family [1].
• E4TF1 was originally identified as one of the transcription factors responsible for adenovirus E4 gene transcription [2].
• Regulation of human B19 parvovirus promoter expression by hGABP (E4TF1) transcription factor [3].
• Promoter activity from a construct without a hGABP/E4TF1-binding site was markedly reduced in A549 human lung adenocarcinoma cells, but not in JKT-1 [4].
• Differential response at the hGABP/E4TF1 site of retinoblastoma gene promoter in human testicular seminoma cells [4].

High impact information on GABPAP

• E4TF1 has been purified to homogeneity from HeLa cells by sequence-specific DNA affinity chromatography and characterized [5].
• This study provides evidence that the interaction between the two different subunits of E4TF1 is required for it to function as a transcription factor, and that one of the subunits binds to a specific DNA sequence and the other works as a modulator [5].
• E4TF1 was purified, and the partial amino acid sequence of each subunit was determined [2].
• The upstream region of the rat H+/K(+)-ATPase beta subunit gene contains direct repeat sequences and palindromes, potential binding sites for RNA polymerase II and E4TF1, and CACCC box sequences [6].
• Structures, sequence characteristics, and synteny relationships of the transcription factor E4TF1, the splicing factor U2AF35 and the cystathionine beta synthetase genes from Fugu rubripes [7].

Biological context of GABPAP

• Regional mapping of two subunits of transcription factor E4TF1 to human chromosome [8].
• Furthermore, promoter activity from a plasmid carrying a point mutation at the hGABP/E4TF1 site was reduced in A549 cells, but not altered in JKT-1 cells [4].
• Several potential transcription regulatory motifs, such as Sp-1, E4TF1 and ATF binding sites, were identified in the 5'-flanking region [9].

References