High impact information on SFI1

• The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled [1].
• The discovery of Sfi1p as a novel binding partner for the Ca2+-binding protein centrin has provided new insight into the dynamic behavior of centrosomes [2].
• We have called it SFI1, for suppressor of fil1 [3].
• SFI1 is an essential gene [3].
• Cells expressing SFI1 under the control of a galactose-inducible promoter arrest after addition of glucose as doublets of undivided mother and daughter cells [3].

Biological context of SFI1

• Hence, Sfi1 might be required for correct mitotic spindle assembly and its precise role might be in chromosome condensation [3].
• Further analysis showed that Sfi1 is required for cell cycle progression, more specifically for progression through G(2)-M transition [3].
• The restriction endonucleases Sma1 and Sfi1 which recognise rare-medium frequency sites in the Arabidopsis genome were used [4].

Anatomical context of SFI1

• We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/CLIP-170 and the SPB half-bridge protein Sfi1p [5].

Analytical, diagnostic and therapeutic context of SFI1

• Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix [1].

References