High impact information on KIN2

• These genes, designated KIN1 and KIN2, are closely related to each other [1].
• Finally, we find that Kin1 and Kin2 induce phosphorylation of t-SNARE Sec9 in vivo and stimulate its release from the plasma membrane [2].
• We isolated KIN1 and KIN2 as suppressors of a mutant form of Rho3, a Rho-GTPase acting in polarized trafficking [2].
• Interestingly, a significant fraction of KIN1 and KIN2 proteins are present in purified cell wall preparations [3].
• In previous studies, we identified the yeast KIN1 gene product (pp145KIN1) and KIN2 gene product (pp145KIN2) as 145 kDa phosphoproteins with serine/threonine protein kinase activity [3].

Biological context of KIN2

• KIN1 and KIN2 are transcribed into 3.5-kilobase mRNAs that contain uninterrupted open reading frames encoding polypeptides of 117 kDa and 126 kDa, respectively [1].
• Two genes, KIN1 and KIN2, were isolated in Saccharomyces cerevisiae by their homology to the protein kinase family of viral oncogenes [3].
• The sequence shows a high level of identity with protein kinases over the amino-terminal catalytic domain, and limited identity with yeast protein kinases SNF1, KIN2 and KIN1 over part of the carboxyl-terminal domain [4].

Other interactions of KIN2

• Here, we present evidence for a novel link between Kin1, Kin2, and the secretory machinery of the budding yeast [2].

References