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Gene Review:

VTA1 - Vta1p

Saccharomyces cerevisiae S288c

Synonyms: VPS20-associated protein 1, Vacuolar protein sorting-associated protein VTA1, YLR181C

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Disease relevance of VTA1

Deletion of VTA1 or VPS60, another putative class E gene, resulted in calcofluor white hypersensitivity [1].

High impact information on VTA1

We characterize Vta1 as a positive regulator of Vps4 both in vivo and in vitro [2].
These results reveal functional roles for the Vps46p and Vta1p proteins in regulating the ESCRT complex assembly/disassembly cycle in protein sorting at the yeast late endosome [3].
Iron-induced degradation of Fet3p-Ftr1p is significantly reduced in strains containing a deletion of a gene, VTA1, which is involved in multivesicular body (MVB) sorting in yeast [4].
Deletion of VTA1 did not affect growth on raffinose and only mildly affected carboxypeptidase S sorting [1].
We identified VTA1 in a screen for mutations that result in altered vacuole morphology [1].

Biological context of VTA1

Deletion of either VPS20 (vps20Delta) or VTA1 (vta1Delta) leads to similar class E Vps- phenotypes resembling those of vps4Delta, including carboxypeptidase Y (CPY) secretion, a block in ubiquitin-dependent MVB sorting, and a delay in both post-internalisation endocytic transport and biosynthetic transport to the vacuole [5].
In a yeast two-hybrid screen with Vps4p as bait we isolated VPS20 (YMR077c) and the novel open reading frame YLR181c, for which the name VTA1 has recently been assigned (Saccharomyces Genome Database) [5].
Both ATP binding [Vps4p-(K179A)] and ATP hydrolysis [Vps4p-(E233Q)] mutant proteins exhibit enhanced binding to Vps20p and Vta1p in vitro [5].
The primary sequence of SBP1 indicates 22.5% identity with that of Vta1p from Saccharomyces cerevisiae, which was recently identified as a novel class E Vps protein binding to Vps4p [6].

Physical interactions of VTA1

Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae [5].

References

Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae. Shiflett, S.L., Ward, D.M., Huynh, D., Vaughn, M.B., Simmons, J.C., Kaplan, J. J. Biol. Chem. (2004) [Pubmed]
Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. Azmi, I., Davies, B., Dimaano, C., Payne, J., Eckert, D., Babst, M., Katzmann, D.J. J. Cell Biol. (2006) [Pubmed]
Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body. Lottridge, J.M., Flannery, A.R., Vincelli, J.L., Stevens, T.H. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
Post-transcriptional regulation of the yeast high affinity iron transport system. Felice, M.R., De Domenico, I., Li, L., Ward, D.M., Bartok, B., Musci, G., Kaplan, J. J. Biol. Chem. (2005) [Pubmed]
Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae. Yeo, S.C., Xu, L., Ren, J., Boulton, V.J., Wagle, M.D., Liu, C., Ren, G., Wong, P., Zahn, R., Sasajala, P., Yang, H., Piper, R.C., Munn, A.L. J. Cell. Sci. (2003) [Pubmed]
Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B. Fujita, H., Umezuki, Y., Imamura, K., Ishikawa, D., Uchimura, S., Nara, A., Yoshimori, T., Hayashizaki, Y., Kawai, J., Ishidoh, K., Tanaka, Y., Himeno, M. J. Cell. Sci. (2004) [Pubmed]

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