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Gene Review:

DAD1 - Dad1p

Saccharomyces cerevisiae S288c

Synonyms: DASH complex subunit DAD1, DUO1 and DAM1-interacting protein 1, Outer kinetochore protein DAD1, YDR016C

Makishima, T. et al., Fu, J. et al., Silberstein, S. et al., Cheeseman, I.M. et al., Enquist-Newman, M. et al.

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High impact information on DAD1

Dam1p, Duo1p, and Dad1p can associate with each other physically and are required for both spindle integrity and kinetochore function in budding yeast [1].
The protein sequence of ost2 mutant alleles revealed mutations at highly conserved residues in the Ost2p/DAD1 protein sequence [2].
In addition, sequence homology between the Ost2 subunit of the yeast OST complex and Dad1 (defender against apoptotic death) suggests that Dad1 may represent a fourth subunit of the mammalian OST complex [3].
The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation [4].
CONCLUSION: tsBN7 cells have a defect in N-linked glycosylation caused by the loss of DAD1 [4].

Biological context of DAD1

Surprisingly, the Ost2 protein was found to be 40% identical to the DAD1 protein (defender against apoptotic cell death), a highly conserved protein initially identified in vertebrate organisms [2].
It has a mutation in the DAD1 gene encoding a 12.5kDa highly conserved protein through evolution, and enters apoptosis at the restrictive temperature due to this mutation [4].

Anatomical context of DAD1

DAD1 was localized in the endoplasmic reticulum [4].
Thus, DAD1 appears to be an integral membrane protein in which both termini are located in the cytosol [4].

Other interactions of DAD1

These results indicate that Dad1p functions as a component of the Duo1p/Dam1p complex [5].

Analytical, diagnostic and therapeutic context of DAD1

RESULTS: DAD1 was recovered in light membrane fractions after differential centrifugation [4].
The yeast two-hybrid assay also revealed affinity between the cytoplasmically located N-terminal region of Dad1 and the short cytoplasmic tail of OST48, thus placing Dad1 firmly into the OST complex [3].

References

Implication of a novel multiprotein Dam1p complex in outer kinetochore function. Cheeseman, I.M., Brew, C., Wolyniak, M., Desai, A., Anderson, S., Muster, N., Yates, J.R., Huffaker, T.C., Drubin, D.G., Barnes, G. J. Cell Biol. (2001) [Pubmed]
The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms. Silberstein, S., Collins, P.G., Kelleher, D.J., Gilmore, R. J. Cell Biol. (1995) [Pubmed]
Interactions among subunits of the oligosaccharyltransferase complex. Fu, J., Ren, M., Kreibich, G. J. Biol. Chem. (1997) [Pubmed]
The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation. Makishima, T., Nakashima, T., Nagata-Kuno, K., Fukushima, K., Iida, H., Sakaguchi, M., Ikehara, Y., Komiyama, S., Nishimoto, T. Genes Cells (1997) [Pubmed]
Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity. Enquist-Newman, M., Cheeseman, I.M., Van Goor, D., Drubin, D.G., Meluh, P.B., Barnes, G. Mol. Biol. Cell (2001) [Pubmed]

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