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Gene Review

OST2  -  Ost2p

Saccharomyces cerevisiae S288c

Synonyms: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2, Oligosaccharyl transferase 16 kDa subunit, Oligosaccharyl transferase subunit OST2, Oligosaccharyl transferase subunit epsilon, YOR103C, ...
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High impact information on OST2

  • The protein sequence of ost2 mutant alleles revealed mutations at highly conserved residues in the Ost2p/DAD1 protein sequence [1].
  • Microsomal membranes isolated from ost2 mutant yeast show marked reductions in the in vitro transfer of high mannose oligosaccharide from exogenous lipid-linked oligosaccharide to a glycosylation site acceptor tripeptide [1].
  • Overexpression of the Ost2 protein suppresses the temperature-sensitive phenotype of the wbp1-2 allele and increases in vivo and in vitro oligosaccharyltransferase activity in a wbp1-2 strain [1].
  • Genomic disruption of the OST2 locus was lethal in haploid yeast showing that expression of the Ost2 protein is essential for viability [1].
  • These findings also suggested that Ost2p, Stt3p, Ost5p, and Ost6p do not have a cleavable N-terminal signal sequence [2].

Biological context of OST2

  • Surprisingly, the Ost2 protein was found to be 40% identical to the DAD1 protein (defender against apoptotic cell death), a highly conserved protein initially identified in vertebrate organisms [1].
  • In addition, sequence homology between the Ost2 subunit of the yeast OST complex and Dad1 (defender against apoptotic death) suggests that Dad1 may represent a fourth subunit of the mammalian OST complex [3].
  • Here we show that ost mutants, such as ost2 and wbp1-1, display morphological and biochemical features of apoptosis upon induction of the glycosylation defect [4].
  • These results suggest that apoptosis-like cell death in ost2 mutants is caused by the secondary effect of overall reduced protein N-linked glycosylation [5].
  • At elevated temperatures, ost2 mutants arrested growth by decreasing cell viability [5].

Associations of OST2 with chemical compounds


  1. The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms. Silberstein, S., Collins, P.G., Kelleher, D.J., Gilmore, R. J. Cell Biol. (1995) [Pubmed]
  2. Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions. Yan, A., Wu, E., Lennarz, W.J. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  3. Interactions among subunits of the oligosaccharyltransferase complex. Fu, J., Ren, M., Kreibich, G. J. Biol. Chem. (1997) [Pubmed]
  4. Defects in N-glycosylation induce apoptosis in yeast. Hauptmann, P., Riel, C., Kunz-Schughart, L.A., Fröhlich, K.U., Madeo, F., Lehle, L. Mol. Microbiol. (2006) [Pubmed]
  5. Yeast cell death caused by mutation of the OST2 gene encoding the epsilon-subunit of Saccharomyces cerevisiae oligosaccharyltransferase. Sugiura, M., Takagi, H. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
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