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Gene Review:

EXG2 - Exg2p

Saccharomyces cerevisiae S288c

Synonyms: Exo-1,3-beta-glucanase 2, Glucan 1,3-beta-glucosidase 2, YD9320A.12C, YDR261C

Esteban, P.F. et al., Baladrón, V. et al., Correa, J. et al., Larriba, G. et al., Larriba, G. et al.

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High impact information on EXG2

The molecular cloning of 1,3-beta-glucanase-encoding genes from different yeast species was achieved by screening genomic libraries with DNA probes obtained by PCR-amplification using oligonucleotides designed according to conserved regions in the EXG1, EXG2 and SSG1 genes from Saccharomyces cerevisiae [1].
Gene EXG2 encodes a 63 kDa polypeptide with 12 potential glycosylation sites [2].
EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique [3].
ENG1 (YNR067c), a gene encoding a new endo-1,3-beta-glucanase, was cloned by screening a genomic library with a DNA probe obtained by PCR with synthetic oligonucleotides designed according to conserved regions found between yeast exo-1,3-beta-glucanases (Exglp, Exg2p, and Ssglp) [4].
A different exoglucanase, encoded by a second gene (EXG2), has been characterized as a heavily glycosylated, membrane bound 200 kDa glycoprotein [5].

Biological context of EXG2

Single or double disruption of EXG1 and EXG2 did not result in any relevant phenotype and the triple mutant behaved as ssg1/ssg1 [2].

References

Cloning and characterization of 1,3-beta-glucanase-encoding genes from non-conventional yeasts. Esteban, P.F., Vazquez de Aldana, C.R., del Rey, F. Yeast (1999) [Pubmed]
Molecular biology of yeast exoglucanases. Larriba, G., Andaluz, E., Cueva, R., Basco, R.D. FEMS Microbiol. Lett. (1995) [Pubmed]
Genetic mapping of 1,3-beta-glucanase-encoding genes in Saccharomyces cerevisiae. Correa, J., Vazquez de Aldana, C.R., San Segundo, P., del Rey, F. Curr. Genet. (1992) [Pubmed]
Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae. Baladrón, V., Ufano, S., Dueñas, E., Martín-Cuadrado, A.B., del Rey, F., Vázquez de Aldana, C.R. Eukaryotic Cell (2002) [Pubmed]
Yeast exoglucanases. Where redundancy implies necessity. Larriba, G., Basco, R.D., Andaluz, E., Luna-Arias, J.P. Arch. Med. Res. (1993) [Pubmed]

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