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Gene Review

SPR1  -  Spr1p

Saccharomyces cerevisiae S288c

Synonyms: Exo-1,3-beta-glucanase, SSG1, Sporulation-specific glucan 1,3-beta-glucosidase, YOR190W
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High impact information on SPR1

  • Mapping and sequence analysis also reveal that the SPR1 mitochondrial genome lacks three sectors of the wild-type molecule of 4.4, 1.7, and 0.5 kilobases [1].
  • Absence of these sectors together with a rearranged gene order does not appear to affect the phenotype of SPR1, as colony morphology and growth rate on a number of different substrates are not detectably different from the wild type [1].
  • Lack of phenotypic change suggests that mitochondrial gene expression has not been noticeably disrupted in SPR1 despite deletion of the consensus nonomer promoter upstream from the glutamic acid tRNA gene [1].
  • The purified enzyme represented only 0.001% of the total 1,3-beta-glucanase activity, the remainder being due to an exo-1,3-beta-glucanase enzyme, and behaved as an acidic glycoprotein (pI 3.3) in isoelectric-focusing experiments [2].
  • We have sequenced the SPR1 gene and found that it has extensive DNA and protein sequence homology to the S. cerevisiae EXG1 gene which encodes an exo-1,3-beta-glucanase expressed during vegetative growth (C. R. Vasquez de Aldana, J. Correa, P. San Segundo, A. Bueno, A. R. Nebrada, E. Mendez, and F. del Ray, Gene 97:173-182, 1991) [3].

Biological context of SPR1

  • The YlEXG1 gene of Yarrowia lipolytica, encoding an exo-1, 3-beta-glucanase, was isolated by screening a genomic library with a DNA probe obtained by PCR amplification, using oligonucleotides designed according to conserved regions in the EXG1, EXG2 and SSG1 genes from Saccharomyces cerevisiae [4].
  • Gene SSG1 encodes a 52 kDa polypeptide which is specifically synthesized during sporulation of diploids [5].
  • The meiotic time course of SSG1 induction indicates that the gene is transcribed only in the late stages of the process, beginning at the time of meiosis I and reaching a maximum during spore formation [6].
  • Nucleotide sequence of the exo-1,3-beta-glucanase-encoding gene, EXG1, of the yeast Saccharomyces cerevisiae [7].
  • Finally, the SSG1 locus mapped on the right arm of chromosome XV, about 8.2 centimorgans to the centromere-proximal side of HIS3 [8].

Associations of SPR1 with chemical compounds


Other interactions of SPR1

  • We conclude that SPR1, SPR2 and SPR3 transcription is modulated during sporulation, possibly in response to earlier events in the process [11].
  • EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique [8].
  • Northern (RNA) analysis reveals a unique SSG1-specific transcript, 1.7 kb long, which can be detected only in sporulating diploids (MATa/MAT alpha) but does not appear in vegetatively growing cells or in nonsporulating diploids (MAT alpha/MAT alpha) when incubated under nitrogen starvation conditions [6].


  1. In vivo rearrangement of mitochondrial DNA in Saccharomyces cerevisiae. Clark-Walker, G.D. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  2. Occurrence of an endo-1,3-beta-glucanase in culture fluids of the yeast Candida utilis. Purification and characterization of the enzyme activity. Villa, T.G., Notario, V., Villanueva, J.R. Biochem. J. (1979) [Pubmed]
  3. The Saccharomyces cerevisiae SPR1 gene encodes a sporulation-specific exo-1,3-beta-glucanase which contributes to ascospore thermoresistance. Muthukumar, G., Suhng, S.H., Magee, P.T., Jewell, R.D., Primerano, D.A. J. Bacteriol. (1993) [Pubmed]
  4. Cloning and characterization of the EXG1 gene from the yeast Yarrowia lipolytica. Esteban, P.F., Casarégola, S., Vazquez De Aldana, C.R., Del Rey, F. Yeast (1999) [Pubmed]
  5. Molecular biology of yeast exoglucanases. Larriba, G., Andaluz, E., Cueva, R., Basco, R.D. FEMS Microbiol. Lett. (1995) [Pubmed]
  6. SSG1, a gene encoding a sporulation-specific 1,3-beta-glucanase in Saccharomyces cerevisiae. San Segundo, P., Correa, J., Vazquez de Aldana, C.R., del Rey, F. J. Bacteriol. (1993) [Pubmed]
  7. Nucleotide sequence of the exo-1,3-beta-glucanase-encoding gene, EXG1, of the yeast Saccharomyces cerevisiae. Vazquez de Aldana, C.R., Correa, J., San Segundo, P., Bueno, A., Nebreda, A.R., Mendez, E., del Rey, F. Gene (1991) [Pubmed]
  8. Genetic mapping of 1,3-beta-glucanase-encoding genes in Saccharomyces cerevisiae. Correa, J., Vazquez de Aldana, C.R., San Segundo, P., del Rey, F. Curr. Genet. (1992) [Pubmed]
  9. Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production. Santos, T., del Rey, F., Conde, J., Villanueva, J.R., Nombela, C. J. Bacteriol. (1979) [Pubmed]
  10. Exo-1,3-beta-glucanase activity in Candida albicans: effect of the yeast-to-mycelium transition. Molina, M., Cenamor, R., Nombela, C. J. Gen. Microbiol. (1987) [Pubmed]
  11. Transcriptional regulation of sporulation genes in yeast. Holaway, B.L., Kao, G., Finn, M.C., Clancy, M.J. Mol. Gen. Genet. (1987) [Pubmed]
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