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Gene Review

DSE4  -  Dse4p

Saccharomyces cerevisiae S288c

Synonyms: Daughter specific expression protein 4, ENG1, Endo-1,3(4)-beta-glucanase 1, Endo-1,3-beta-glucanase 1, Endo-1,4-beta-glucanase 1, ...
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Disease relevance of DSE4


High impact information on DSE4

  • Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of > 300 kDa [2].
  • The endo-1,3-beta-glucanase (EC secreted into the culture medium by cells of Candida utilis was isolated and purified to homogeneity on polyacrylamide-gel electrophoresis and in ultracentrifugation studies (s20,w = 1.97S) [3].
  • Treatments with buffered solutions at pH 6.0 during growth of A. alternata in the presence of glucose derepressed AaK1 expression and endo-1,4-beta-glucanase production and enhanced decay development on the fruit [4].
  • Saccharomyces cerevisiae S288C produced two laminarinases (1,3-beta-glucanases) which were separated by diethylaminoethyl-Sephadex column chromatography; one was an endo-1,3-beta-glucanase, and the other was an exo-1,3-beta-glucanase active not only on laminarin but also on pustulan (1,6-beta-glucan) and on p-nitrophenyl-beta-D-glucoside [5].
  • In fks1Delta and gsc2/fks2Delta cells, defective in beta-1,3-glucan synthesis, modification of the protein pattern found in the supernatant of the growth medium, as well as the material released by beta-ME or laminarinase, was evident [6].

Biological context of DSE4


Associations of DSE4 with chemical compounds

  • Eng1p and Acf2p/Eng2p are glucan-hydrolyzing proteins that specifically act on 1,3-beta linkages, with an endolytic mode of action [7].
  • From the phosphate-labeled glycoprotein fraction released by laminarinase, three cell wall mannoproteins, Ccw12p, Ccw13p and Ccw14p, were isolated and identified by N-terminal sequencing [9].
  • A small amount could be extracted with SDS, but most of it could only be extracted with laminarinase [10].
  • In this present study, to increase the yield of water-soluble beta-glucan, the wild type of Sacharomyces cerevisiae, JH, was treated with a combination of UV irradiation and laminarinase (endo-beta-(1,3)-glucanase) to yield the laminarinase-resistant mutants, JUL1 and JUL3 [11].
  • The transglycosylation of p-nitrophenyl-beta-D-cellotrioside to cellotetraose catalyzed by endo-1,4-beta-glucanase (cellulase, EC from a psychrotrophic yeast, Rhodotorula glutinis KUJ 2731, was increased by addition of a miscible organic solvent in the reaction mixture [12].

Other interactions of DSE4

  • The MHP1 gene product is a predicted 1398 amino acid protein and only approximately 80% of the amino portion of this protein is required for laminarinase resistance [8].
  • A pbs2 delta disruption strain was laminarinase super-sensitive and supersensitive to K1 killer toxin while a strain carrying PBS2 at multiple copy was resistant to killer toxin [8].
  • In the CCW11 disruption the Ccw11p as well as the laminarinase-extracted Ccw5 protein was missing [13].

Analytical, diagnostic and therapeutic context of DSE4


  1. The binding pattern of two carbohydrate-binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in beta-glucan binding within family CBM4. Zverlov, V.V., Volkov, I.Y., Velikodvorskaya, G.A., Schwarz, W.H. Microbiology (Reading, Engl.) (2001) [Pubmed]
  2. Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins. Hagen, I., Ecker, M., Lagorce, A., Francois, J.M., Sestak, S., Rachel, R., Grossmann, G., Hauser, N.C., Hoheisel, J.D., Tanner, W., Strahl, S. Mol. Microbiol. (2004) [Pubmed]
  3. Occurrence of an endo-1,3-beta-glucanase in culture fluids of the yeast Candida utilis. Purification and characterization of the enzyme activity. Villa, T.G., Notario, V., Villanueva, J.R. Biochem. J. (1979) [Pubmed]
  4. pH regulates endoglucanase expression and virulence of Alternaria alternata in persimmon fruit. Eshel, D., Miyara, I., Ailing, T., Dinoor, A., Prusky, D. Mol. Plant Microbe Interact. (2002) [Pubmed]
  5. Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production. Santos, T., del Rey, F., Conde, J., Villanueva, J.R., Nombela, C. J. Bacteriol. (1979) [Pubmed]
  6. Functional analysis of the cysteine residues and the repetitive sequence of Saccharomyces cerevisiae Pir4/Cis3: the repetitive sequence is needed for binding to the cell wall beta-1,3-glucan. Castillo, L., Martinez, A.I., Garcerá, A., Elorza, M.V., Valentín, E., Sentandreu, R. Yeast (2003) [Pubmed]
  7. Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae. Baladrón, V., Ufano, S., Dueñas, E., Martín-Cuadrado, A.B., del Rey, F., Vázquez de Aldana, C.R. Eukaryotic Cell (2002) [Pubmed]
  8. Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae. Lai, M.H., Silverman, S.J., Gaughran, J.P., Kirsch, D.R. Yeast (1997) [Pubmed]
  9. Deletion of new covalently linked cell wall glycoproteins alters the electrophoretic mobility of phosphorylated wall components of Saccharomyces cerevisiae. Mrsa, V., Ecker, M., Strahl-Bolsinger, S., Nimtz, M., Lehle, L., Tanner, W. J. Bacteriol. (1999) [Pubmed]
  10. Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Schreuder, M.P., Brekelmans, S., van den Ende, H., Klis, F.M. Yeast (1993) [Pubmed]
  11. High-level TNF-alpha secretion and macrophage activity with soluble beta-glucans from Saccharomyces cerevisiae. Lee, D.Y., Ji, I.H., Chang, H.I., Kim, C.W. Biosci. Biotechnol. Biochem. (2002) [Pubmed]
  12. Increased transglycosylation activity of Rhodotorula glutinis endo-beta-glucanase in media containing organic solvent. Oikawa, T., Tsukagawa, Y., Chino, M., Soda, K. Biosci. Biotechnol. Biochem. (2001) [Pubmed]
  13. Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Mrsă, V., Seidl, T., Gentzsch, M., Tanner, W. Yeast (1997) [Pubmed]
  14. Glucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain. Montijn, R.C., van Rinsum, J., van Schagen, F.A., Klis, F.M. J. Biol. Chem. (1994) [Pubmed]
  15. Cell Surface Expression of Bacterial Esterase A by Saccharomyces cerevisiae and Its Enhancement by Constitutive Activation of the Cellular Unfolded Protein Response. Breinig, F., Diehl, B., Rau, S., Zimmer, C., Schwab, H., Schmitt, M.J. Appl. Environ. Microbiol. (2006) [Pubmed]
  16. Cloning and expression of a novel Trichoderma viride laminarinase AI gene (lamAI). Nobe, R., Sakakibara, Y., Ogawa, K., Suiko, M. Biosci. Biotechnol. Biochem. (2004) [Pubmed]
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