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Gene Review

SSN2  -  Ssn2p

Saccharomyces cerevisiae S288c

Synonyms: MED13, Mediator complex subunit 13, Mediator of RNA polymerase II transcription subunit 13, NUT8, Protein SCA1, ...
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High impact information on SSN2

  • Genetic and biochemical data indicate that Srb9p is a substrate for PKA and that this phosphorylation modulates the activity of the Srb complex [1].
  • Two components of the RNA polymerase II machinery, Med13 (Srb9) and Spt8, were isolated as two-hybrid partners of the conserved TFIIS N-terminal domain [2].
  • Moreover, LexA-Sfl1 represses transcription of a reporter, and repression is reduced in an srb9 mutant [3].
  • Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator [4].
  • Furthermore, the association of Srb9 with the GAL1 upstream activation sequence requires SAGA and specifically Spt3 [5].

Biological context of SSN2

  • In contrast to the srb9 suppressor mutation that we identified, an srb9Delta mutation causes a strong defect in Gal4-activated transcription [5].
  • Genetic epistasis studies suggest that the UME2 and UME5 gene products act in the same pathway to regulate meiotic transcript stability [6].
  • We developed a selection system for isolating suppressors of this lethal phenotype and cloned a gene, SCA1 (suppressor of CTD alanine), which complements recessive suppressors of lethal multiple-substitution mutations [7].

Associations of SSN2 with chemical compounds

  • A partial deletion of SCA1 (sca1 delta ::hisG) suppresses alanine or glutamate substitutions at position two of the consensus CTD sequence, and a lethal CTD truncation mutation, but SCA1 deletion does not suppress alanine or glutamate substitutions at position five [7].

Other interactions of SSN2

  • We demonstrate the utility of this technique by isolating three genes, GAL83, SSN2 and MAK7, each of which presents one of these problems for cloning [8].
  • Finally, Srb9 association also requires TBP [5].
  • We obtained eight complementation groups and identified the genes corresponding to three of the groups as NHP10, HDA1, and SRB9 [5].
  • Two genes, UME2 and UME5, that regulate the stability of meiosis-specific transcripts have been identified [6].


  1. The Ras/PKA signaling pathway directly targets the Srb9 protein, a component of the general RNA polymerase II transcription apparatus. Chang, Y.W., Howard, S.C., Herman, P.K. Mol. Cell (2004) [Pubmed]
  2. Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS. Wery, M., Shematorova, E., Van Driessche, B., Vandenhaute, J., Thuriaux, P., Van Mullem, V. EMBO J. (2004) [Pubmed]
  3. Srb/mediator proteins interact functionally and physically with transcriptional repressor Sfl1. Song, W., Carlson, M. EMBO J. (1998) [Pubmed]
  4. TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. Samuelsen, C.O., Baraznenok, V., Khorosjutina, O., Spahr, H., Kieselbach, T., Holmberg, S., Gustafsson, C.M. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  5. The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Larschan, E., Winston, F. Mol. Cell. Biol. (2005) [Pubmed]
  6. The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose. Surosky, R.T., Strich, R., Esposito, R.E. Mol. Cell. Biol. (1994) [Pubmed]
  7. Suppression analysis reveals a functional difference between the serines in positions two and five in the consensus sequence of the C-terminal domain of yeast RNA polymerase II. Yuryev, A., Corden, J.L. Genetics (1996) [Pubmed]
  8. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Erickson, J.R., Johnston, M. Genetics (1993) [Pubmed]
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