Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

SFL1 - Sfl1p

Saccharomyces cerevisiae S288c

Synonyms: Flocculation suppression protein, Protein SFL1, YOR140W, YOR3339W

Kim, T.S. et al., Conlan, R.S. et al., Tonouchi, A. et al., Fujita, A. et al., Song, W. et al., et al.

Conlan, Tzamarias,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on SFL1

Moreover, LexA-Sfl1 represses transcription of a reporter, and repression is reduced in an srb9 mutant [1].
Sfl1 acts on SUC2 through a repression site located immediately 5' to the TATA box, and Sfl1 binds this DNA sequence in vitro [1].
NRG1 and SFL1 expression requires the Srb8-11 complex, and correspondingly, the Srb8-11 complex is also necessary for STA1 repression [2].
Here we demonstrate that glucose-dependent repression of STA1 expression is imposed by both Sfl1 and Nrg1, which serve as direct transcriptional repressors [2].
In addition, we show that the levels of Nrg1 and Sfl1 increase in glucose-grown cells, suggesting that the effects of glucose are mediated, at least in part, through an increase in the abundance of these repressors [2].

Biological context of SFL1

An active ribozyme sequence, targeted for the SFL1 gene (a yeast suppressor gene for flocculation) was fused just downstream of the T7 promoter [3].
These data indicate a possible mechanism for regulating Sfl1-mediated repression through modulation of DNA binding by cAMP-dependent protein kinase-dependent phosphorylation [4].

Physical interactions of SFL1

In addition, the N-terminal region of the SFL1 gene product shows extensive homology to the DNA-binding domain of HSTF [5].
Sfl1 is known to interact with Tpk2, a cAMP-dependent protein kinase that negatively regulates Sfl1 function [4].

Other interactions of SFL1

These results indicate a possible mechanism for Sfl1-mediated repression via Ssn6-Tup1 and specific subunits of the RNA polymerase II holoenzyme [4].
Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor [5].
We identified a yeast gene encoding Flocculent Specific Protein (FSP) produced excessively in the SFL1 gene-disrupted flocculent strain [6].

Analytical, diagnostic and therapeutic context of SFL1

Molecular cloning of the gene encoding a highly expressed protein in SFL1 gene-disrupted flocculating yeast [6].

References

Srb/mediator proteins interact functionally and physically with transcriptional repressor Sfl1. Song, W., Carlson, M. EMBO J. (1998) [Pubmed]
Glucose repression of STA1 expression is mediated by the Nrg1 and Sfl1 repressors and the Srb8-11 complex. Kim, T.S., Lee, S.B., Kang, H.S. Mol. Cell. Biol. (2004) [Pubmed]
Construction of a novel artificial-ribozyme-releasing plasmid. Taira, K., Oda, M., Shinshi, H., Maeda, H., Furukawa, K. Protein Eng. (1990) [Pubmed]
Sfl1 functions via the co-repressor Ssn6-Tup1 and the cAMP-dependent protein kinase Tpk2. Conlan, R.S., Tzamarias, D. J. Mol. Biol. (2001) [Pubmed]
Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor. Fujita, A., Kikuchi, Y., Kuhara, S., Misumi, Y., Matsumoto, S., Kobayashi, H. Gene (1989) [Pubmed]
Molecular cloning of the gene encoding a highly expressed protein in SFL1 gene-disrupted flocculating yeast. Tonouchi, A., Fujita, A., Kuhara, S. J. Biochem. (1994) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.