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Gene Review

GNP1  -  glutamine permease GNP1

Saccharomyces cerevisiae S288c

Synonyms: D9719.14, High-affinity glutamine permease, YDR508C
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High impact information on GNP1

  • Direct immunofluorescence showed large increases in the expression of Gnp1 and Bap2 proteins when grown in galactose compared with glucose medium [1].
  • In contrast to Agp1p, Gnp1p is not functionally expressed when cells are grown on media containing proline as sole nitrogen source [2].
  • Helix III was affected in both CAN1 (Y173H, Y173D) and GNP1 (W239C) mutants and has previously been found to be important for substrate preference in other members of the family [3].
  • Constitutive overexpression of the genes for glutamine permease ( GNP1) or methionine permease ( MUP1) enhanced the assimilation of glutamine or methionine in the ssy1Delta strain but could not fully suppress the derepression of the NCR-sensitive genes or MET genes [4].
  • While Gap1p and Agp1p appear to be the main cysteine transporters on the non-repressing nitrogen source proline, Bap2p, Bap3p, Tat1p, Tat2p, Agp1p and Gnp1p are all important for cysteine uptake on ammonium-based medium [5].

Biological context of GNP1


Associations of GNP1 with chemical compounds

  • The Km of GNP1 for glutamine uptake was determined to be 0.59 mM [6].
  • Gnp1p, which is closely related to Agp1p, has a somewhat less-broad specificity, transporting Leu, Ser, Thr, Cys, Met, Gln and Asn, while Bap2p and Bap3p, which are also closely related to Agp1p, are able to transport Ile, Leu, Val, Cys, Met, Phe, Tyr and Trp [7].

Other interactions of GNP1

  • Furthermore, whereas Bap2p, Bap3p, Tat1p and Tat2p seem most important under amino acid-rich conditions, Agp1p contributes significantly when only ammonium is present, and Gnp1p only contributes under the latter condition [5].


  1. Carbon catabolite repression regulates amino acid permeases in Saccharomyces cerevisiae via the TOR signaling pathway. Peter, G.J., Düring, L., Ahmed, A. J. Biol. Chem. (2006) [Pubmed]
  2. Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast. Andréasson, C., Neve, E.P., Ljungdahl, P.O. Yeast (2004) [Pubmed]
  3. Amino acid residues important for substrate specificity of the amino acid permeases Can1p and Gnp1p in Saccharomyces cerevisiae. Regenberg, B., Kielland-Brandt, M.C. Yeast (2001) [Pubmed]
  4. Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae. Kodama, Y., Omura, F., Takahashi, K., Shirahige, K., Ashikari, T. Curr. Genet. (2002) [Pubmed]
  5. Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases. Düring-Olsen, L., Regenberg, B., Gjermansen, C., Kielland-Brandt, M.C., Hansen, J. Curr. Genet. (1999) [Pubmed]
  6. GNP1, the high-affinity glutamine permease of S. cerevisiae. Zhu, X., Garrett, J., Schreve, J., Michaeli, T. Curr. Genet. (1996) [Pubmed]
  7. Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae. Regenberg, B., Düring-Olsen, L., Kielland-Brandt, M.C., Holmberg, S. Curr. Genet. (1999) [Pubmed]
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