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AGP1  -  amino acid transporter AGP1

Saccharomyces cerevisiae S288c

Synonyms: Asparagine/glutamine permease, General amino acid permease AGP1, YCL025C, YCL25C
 
 
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High impact information on AGP1

  • In the yck mutant lacking this kinase, Stp1 is not cleaved and AGP1 is not induced in response to amino acids [1].
  • Saccharomyces cerevisiae cells possess a plasma membrane sensor able to detect the presence of extracellular amino acids and then to activate a signaling pathway leading to transcriptional induction of multiple genes, e.g., AGP1, encoding an amino acid permease [1].
  • It was surprising that the same residues were not important for the role of Grr1 in nutrient-regulated transcription of HXT1 or AGP1 [2].
  • We show here that SSY1 is required for transcriptional induction, in response to multiple amino acids, of the AGP1 gene encoding a low-affinity, broad-specificity amino acid permease [3].
  • Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease [3].
 

Biological context of AGP1

 

Associations of AGP1 with chemical compounds

  • Ssy5 is significantly similar to serine proteases, its self-processing is a prerequisite for Stp1 cleavage, and its overexpression causes inducer-independent Stp1 cleavage and high-level AGP1 transcription [1].
  • Consistent with a role for Ssy1p in the recognition of amino acids, a mutant form of the protein with a Thr to Ile substitution in the eighth predicted transmembrane domain is competent for the induction of AGP1 by leucine but not by other amino acids [5].
  • Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression [6].
  • Conversely, AGP1 is strongly induced by tryptophan in a mutant strain largely deficient in tryptophan uptake, but it remains unexpressed in a mutant that accumulates high levels of tryptophan endogenously [3].
  • The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine [6].
 

Other interactions of AGP1

  • In a screen for other mutants defective in the Ssy1p pathway, we confirmed that PTR3 and SSY5 encode additional factors essential for AGP1 expression in response to multiple amino acids [5].
  • Nitrogen-source control of AGP1 induction is mediated by the GATA factor Gln3, likely acting through adjacent 5'-GATA-3' sequences, to amplify the positive effect of UAS(AA) [7].
  • In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2 [8].

References

  1. Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Abdel-Sater, F., El Bakkoury, M., Urrestarazu, A., Vissers, S., André, B. Mol. Cell. Biol. (2004) [Pubmed]
  2. F-box protein Grr1 interacts with phosphorylated targets via the cationic surface of its leucine-rich repeat. Hsiung, Y.G., Chang, H.C., Pellequer, J.L., La Valle, R., Lanker, S., Wittenberg, C. Mol. Cell. Biol. (2001) [Pubmed]
  3. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Iraqui, I., Vissers, S., Bernard, F., de Craene, J.O., Boles, E., Urrestarazu, A., André, B. Mol. Cell. Biol. (1999) [Pubmed]
  4. Grr1p is required for transcriptional induction of amino acid permease genes and proper transcriptional regulation of genes in carbon metabolism of Saccharomyces cerevisiae. Eckert-Boulet, N., Regenberg, B., Nielsen, J. Curr. Genet. (2005) [Pubmed]
  5. Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Bernard, F., André, B. Mol. Microbiol. (2001) [Pubmed]
  6. The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine. Schreve, J.L., Sin, J.K., Garrett, J.M. J. Bacteriol. (1998) [Pubmed]
  7. The external amino acid signaling pathway promotes activation of Stp1 and Uga35/Dal81 transcription factors for induction of the AGP1 gene in Saccharomyces cerevisiae. Abdel-Sater, F., Iraqui, I., Urrestarazu, A., André, B. Genetics (2004) [Pubmed]
  8. Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Bernard, F., André, B. FEBS Lett. (2001) [Pubmed]
 
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