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Gene Review:

SLD3 - Sld3p

Saccharomyces cerevisiae S288c

Synonyms: DNA replication regulator SLD3, G2980, YGL113W

Kamimura, Y. et al., Kanemaki, M. et al., Tanaka, S. et al., Takayama, Y. et al.

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High impact information on SLD3

Therefore, Dpb11, Sld3, Cdc45, and GINS assemble in a mutually dependent manner on replication origins to initiate DNA synthesis [1].
Unlike Sld3, GINS is not required for the initial recruitment of Cdc45 to origins and instead is necessary for stable engagement of Cdc45 with the nascent replisome [2].
Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks [2].
After the establishment of DNA replication forks from early origins, Sld3 is no longer essential for the completion of chromosome replication [2].
Consistently, Sld3 and Cdc45 associate simultaneously with replication origins in the chromatin immunoprecipitation assay: both proteins associate with early-firing origins in G(1) phase and with late-firing origins in late S phase [3].

Biological context of SLD3

Therefore, the origin association of Sld3-Cdc45 complex is prerequisite for origin unwinding in the initiation of DNA replication [3].
These results suggest that the Sld3-Cdc45 complex associates with replication origins through Mcm proteins [3].
Here we show that throughout the cell cycle in Saccharomyces cerevisiae, Cdc45 forms a complex with a novel factor, Sld3 [3].
Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication [4].

Other interactions of SLD3

CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast [4].

References

GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., Araki, H. Genes Dev. (2003) [Pubmed]
Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks. Kanemaki, M., Labib, K. EMBO J. (2006) [Pubmed]
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. Kamimura, Y., Tak, Y.S., Sugino, A., Araki, H. EMBO J. (2001) [Pubmed]
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Tanaka, S., Umemori, T., Hirai, K., Muramatsu, S., Kamimura, Y., Araki, H. Nature (2007) [Pubmed]

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AGOS_AFL019C	Ashbya gossypii ATCC 10895
KLLA0F10681g	Kluyveromyces lactis NRRL Y-1140

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