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Gene Review:

SLD2 - Sld2p

Saccharomyces cerevisiae S288c

Synonyms: DNA replication and checkpoint protein 1, DNA replication regulator SLD2, DRC1, YKL108W, YKL463

Wang, H. et al., Tanaka, S. et al., Masumoto, H. et al., Tak, Y.S. et al., Kamimura, Y. et al.

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High impact information on SLD2

Because this complex is suggested to be essential for chromosomal DNA replication, it seems likely that S-Cdk positively regulates formation of the Sld2-Dpb11 complex and, consequently, chromosomal DNA replication [1].
S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast [1].
A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11 [2].
Simultaneous alanine substitution for serine or threonine in all the canonical CDK-phosphorylation motifs severely reduces complex formation between Sld2 and Dpb11, and inhibits DNA replication [2].
DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae [3].

Biological context of SLD2

DRC1 is an essential cell cycle-regulated gene required for DNA replication [3].
The Drc1 and Dpb11 proteins physically associate and function together to coordinate DNA replication and the cell cycle [3].
We show that both Dpb11 and Drc1 are required for the S-phase checkpoint, including the proper activation of the Rad53 kinase in response to DNA damage and replication blocks [3].
In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication [4].

Physical interactions of SLD2

Immunoprecipitation experiments confirmed that Dpb11 physically interacts with Sld2. sld2-6 cells were defective in DNA replication at the restrictive temperature, as were dpb11-1 cells [5].

Other interactions of SLD2

CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast [4].

Analytical, diagnostic and therapeutic context of SLD2

The interaction between Sld2 and Dpb11 was detected in a two-hybrid assay [5].

References

S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast. Masumoto, H., Muramatsu, S., Kamimura, Y., Araki, H. Nature (2002) [Pubmed]
A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11. Tak, Y.S., Tanaka, Y., Endo, S., Kamimura, Y., Araki, H. EMBO J. (2006) [Pubmed]
DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Wang, H., Elledge, S.J. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Tanaka, S., Umemori, T., Hirai, K., Muramatsu, S., Kamimura, Y., Araki, H. Nature (2007) [Pubmed]
Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Kamimura, Y., Masumoto, H., Sugino, A., Araki, H. Mol. Cell. Biol. (1998) [Pubmed]

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AGOS_ABL045W	Ashbya gossypii ATCC 10895
KLLA0F13794g	Kluyveromyces lactis NRRL Y-1140

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