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Gene Review:

ERG4 - delta(24(24(1)))-sterol reductase

Saccharomyces cerevisiae S288c

Synonyms: C-24(28) sterol reductase, Delta(24(24(1)))-sterol reductase, Sterol Delta(24(28))-reductase, YGL012W, YGL022

Zweytick, D. et al., Smith, S., Sauer, B., Mukhopadhyay, K. et al.

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Disease relevance of ERG4

An erg4 mutant strain exhibited pleiotropic defects such as hypersensitivity to divalent cations and a number of drugs such as cycloheximide, miconazole, 4-nitroquinoline, fluconazole, and sodium dodecyl sulfate [1].

High impact information on ERG4

Recombination events at a loxP site 5' to the structural gene restore expression of YGL022 and result in a multiple drug resistant phenotype [2].
Most of the erg mutants except mutant erg4 were, however, resistant to fluconazole (FLC) [3].
The yeast ERG4 gene encodes sterol C-24(28) reductase which catalyzes the final step in the biosynthesis of ergosterol [1].
Deletion of ERG4 resulted in a complete lack of ergosterol and accumulation of the precursor ergosta-5,7,22,24(28)-tetraen-3beta-ol [1].
Similar to erg6 mutants, erg4 mutants are sensitive to the Golgi-destabilizing drug brefeldin A [1].

Anatomical context of ERG4

Enzyme activity measurements with isolated subcellular fractions revealed that Erg4p is localized to the endoplasmic reticulum [1].
The deduced aa sequence of Sp erg24 shows significant homology with Saccharomyces cerevisiae (Sc) Erg24p, as well as with other members of a larger gene family that includes yeast C-24(28) sterol reductase (Erg4p) and a vertebrate inner nuclear membrane protein, the lamin B receptor (LBR) [4].

References

Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae. Zweytick, D., Hrastnik, C., Kohlwein, S.D., Daum, G. FEBS Lett. (2000) [Pubmed]
Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance. Sauer, B. J. Mol. Biol. (1992) [Pubmed]
Drug susceptibilities of yeast cells are affected by membrane lipid composition. Mukhopadhyay, K., Kohli, A., Prasad, R. Antimicrob. Agents Chemother. (2002) [Pubmed]
Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe. Smith, S. Gene (1995) [Pubmed]

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KLLA0A05071g	Kluyveromyces lactis NRRL Y-1140
MGG_02465	Magnaporthe oryzae 70-15
NCU01333	Neurospora crassa OR74A
sts1	Schizosaccharomyces pombe 972h-

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