High impact information on ERG24

• The sterol C-14 reductase, encoded by the ERG24 gene, could be an effective target for drug development since the morpholine antifungals, inhibitors of Erg24p, have been successful in agricultural applications [1].
• Unlike S. cerevisiae, the C. albicans ERG24 gene was not required for growth, but erg24 mutants showed several altered phenotypes [1].
• Our results indicate that ignosterol can be a suitable sterol for aerobic growth of S. cerevisiae on synthetic media and that inactivation of ERG24 is only conditionally lethal [2].
• The deduced aa sequence of Sp erg24 shows significant homology with Saccharomyces cerevisiae (Sc) Erg24p, as well as with other members of a larger gene family that includes yeast C-24(28) sterol reductase (Erg4p) and a vertebrate inner nuclear membrane protein, the lamin B receptor (LBR) [3].
• The nucleotide sequence of Sp erg24 contains an open reading frame encoding a 424-amino-acid protein [3].

Biological context of ERG24

• We demonstrate that erg2 and erg24 mutants are viable in the deletion consortium background but are lethal when combined in the same haploid strain [4].
• The wild-type ERG24 gene was cloned by complementation onto a 12-kb fragment from a yeast genomic library, and subsequently subcloned onto a 2.4-kb fragment [5].

Associations of ERG24 with chemical compounds

• ERG2 and ERG24 are yeast sterol biosynthetic genes which are targets of morpholine antifungal compounds [4].
• We also demonstrate that the suppressed elo3erg2erg24 does not have a sterol composition markedly different from that of erg24 [4].
• Sterol analysis of the disrupted mutant demonstrated the accumulation of ignosterol, indicating a loss of Erg24p activity [6].
• Tests with a panel of fungicides indicated that pML100 conferred significant resistance only to compounds (Fp, tridemorph, fenpropidin and azasterol) which have a shared site of action, Erg24p [6].
• The Saccharomyces cerevisiae ERG24 gene, encoding sterol delta 14 reductase (Erg24p), was cloned by selecting strains carrying sequences on a 2 mu-based vector for resistance to the morpholine fungicide, fenpropimorph (Fp) [6].

Other interactions of ERG24

• ERG2 is regarded as a non-essential gene but the viability of ERG24 depends on genetic background, type of medium, and CaCl(2) concentration [4].
• Expression was increased two- to fourfold in erg11, erg6 and erg24 backgrounds [7].
• C. albicans erg6 and erg24 mutants were also employed along with the inhibitors, itraconazole and zaragozic acid A, to characterize ERG27 expression using Northern analysis [7].
• A sterol C-14 reductase (erg24-1) mutant of Saccharomyces cerevisiae was selected in a fen1, fen2, suppressor background on the basis of nystatin resistance and ignosterol (ergosta-8,14-dienol) production [5].

Analytical, diagnostic and therapeutic context of ERG24

• Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe [3].
• The sterol C-14 reductase encoded by the Neurospora crassa erg-3 gene: essential charged and polar residues identified by site-specific mutagenesis [8].

References