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Gene Review

PHO86  -  Pho86p

Saccharomyces cerevisiae S288c

Synonyms: Inorganic phosphate transporter PHO86, J0744, YJL117W
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High impact information on PHO86

  • Moreover, Pho84p is localized to the endoplasmic reticulum (ER) and fails to be targeted to the plasma membrane in the absence of Pho86p [1].
  • The other three genes, GTR1, PHO86 and PHO87, are also suggested to be involved in the P(i) uptake system [2].
  • PHO86 has two putative binding sites for the transcriptional activator, Pho4p, at nucleotide positions -191 and -497 relative to the ATG start codon, and showed substantial levels of transcription under high-P(i) conditions and more enhanced levels in low-P(i) medium [2].
  • Both disruption and high dosage of PHO88 or PHO86 resulted in reduced Pi uptake [3].
  • Increased dosage of PHO86, a gene encoding a putative membrane protein associated with a Pi transporter complex, activates the Pi-inhibited Pho81p produced under the control of the GAL1 promoter [3].

Biological context of PHO86

  • The constitutive rAPase+ phenotype of the pho86 pho87 mutant was partially suppressed by an increased dosage of the PHO84 gene [4].

Anatomical context of PHO86


Associations of PHO86 with chemical compounds

  • Deletion of PHO86 in an ino1Delta strain resulted in faster growth when either phosphatidylinositol or glycerophosphoinositol was supplied as the sole inositol source [5].
  • The delta pho86 delta pho88 double disruption resulted in enhanced synthesis of rAPase under the high-Pi condition and conferred arsenate resistance on the cells than those in single disruptants of these genes [3].
  • A genetic screen identified Pho86p, which is required for targeting of the major phosphate transporter (Pho84p) to the plasma membrane, as affecting the utilization of phosphatidylinositol and glycerophosphoinositol [5].

Other interactions of PHO86

  • This mutant has double mutations designated as pho86 and pho87 [4].
  • These and the other findings suggest that the Pho86p and Pho87p proteins collaborate with Pho84p in Pi uptake [4].

Analytical, diagnostic and therapeutic context of PHO86

  • Western blotting analysis of cell extracts revealed that Pho86p, tagged with c-Myc, was fractionated into a water-insoluble fraction [2].


  1. Pho86p, an endoplasmic reticulum (ER) resident protein in Saccharomyces cerevisiae, is required for ER exit of the high-affinity phosphate transporter Pho84p. Lau, W.T., Howson, R.W., Malkus, P., Schekman, R., O'Shea, E.K. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  2. A putative new membrane protein, Pho86p, in the inorganic phosphate uptake system of Saccharomyces cerevisiae. Yompakdee, C., Bun-ya, M., Shikata, K., Ogawa, N., Harashima, S., Oshima, Y. Gene (1996) [Pubmed]
  3. A putative membrane protein, Pho88p, involved in inorganic phosphate transport in Saccharomyces cerevisiae. Yompakdee, C., Ogawa, N., Harashima, S., Oshima, Y. Mol. Gen. Genet. (1996) [Pubmed]
  4. Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Bun-ya, M., Shikata, K., Nakade, S., Yompakdee, C., Harashima, S., Oshima, Y. Curr. Genet. (1996) [Pubmed]
  5. Inositol and phosphate regulate GIT1 transcription and glycerophosphoinositol incorporation in Saccharomyces cerevisiae. Almaguer, C., Mantella, D., Perez, E., Patton-Vogt, J. Eukaryotic Cell (2003) [Pubmed]
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