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Gene Review

PHO81  -  Pho81p

Saccharomyces cerevisiae S288c

Synonyms: CDK inhibitor PHO81, G8567, Phosphate system positive regulatory protein PHO81, YGR233C
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High impact information on PHO81


Biological context of PHO81


Associations of PHO81 with chemical compounds

  • An in vitro assay showed that a glutathione S-transferase-Pho81p fusion protein inhibits the Pho85p protein kinase [9].
  • Interestingly, both read-outs depend on Pho81 but, while the previously described minimum domain of Pho81 is sufficient to sustain phosphate-regulated transcription of PHO genes, full-length Pho81 is required to control trehalose metabolism and the PDS targets [10].

Physical interactions of PHO81

  • However, an in vivo binding assay indicates that the mutant Pho81p is deficient in binding to the Pho80p-Pho85p kinase complex [7].
  • Additionally, we show that Pcl7 interacts with the phosphate-regulated cyclin-cdk inhibitor Pho81, and that the regulation of the Pcl7-Pho85 complex in response to changes in phosphate levels is dependent on Pho81 [11].
  • These findings suggest that Pho88p is also involved in Pi transport and modulates Pho81p function together with Pho86p [12].

Enzymatic interactions of PHO81


Other interactions of PHO81

  • The resulting mutations were introduced into a yeast strain containing a deletion of PHO81 and the effect of the mutation on PHO5 expression was assayed [7].
  • The PHO81 gene is thought to encode an inhibitor of the negative regulators (Pho80p and Pho85p) in the phosphatase (PHO) regulon [9].
  • Elimination of the PHO81 promoter or its substitution by the GAL1 promoter revealed that stimulation of the PHO regulatory system requires both increased transcription of PHO81 and a Pi starvation signal [9].
  • A coordinate increase of PHO80 cancels the dosage effect of PHO4, but not that of PHO81 [13].
  • The PHO81 gene encoding one of the regulators of the phosphatase regulon in Saccharomyces cerevisiae was mapped 9.8 centimorgans distal from the ser2 locus on the right arm of chromosome VII [8].


  1. Phosphate-regulated inactivation of the kinase PHO80-PHO85 by the CDK inhibitor PHO81. Schneider, K.R., Smith, R.L., O'Shea, E.K. Science (1994) [Pubmed]
  2. Signaling phosphate starvation. Lenburg, M.E., O'Shea, E.K. Trends Biochem. Sci. (1996) [Pubmed]
  3. The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal. Hirst, K., Fisher, F., McAndrew, P.C., Goding, C.R. EMBO J. (1994) [Pubmed]
  4. Functional analysis of the cyclin-dependent kinase inhibitor Pho81 identifies a novel inhibitory domain. Huang, S., Jeffery, D.A., Anthony, M.D., O'Shea, E.K. Mol. Cell. Biol. (2001) [Pubmed]
  5. A genetic study of signaling processes for repression of PHO5 transcription in Saccharomyces cerevisiae. Lau, W.W., Schneider, K.R., O'Shea, E.K. Genetics (1998) [Pubmed]
  6. An essential function of a phosphoinositide-specific phospholipase C is relieved by inhibition of a cyclin-dependent protein kinase in the yeast Saccharomyces cerevisiae. Flick, J.S., Thorner, J. Genetics (1998) [Pubmed]
  7. Regulation by phosphorylation of Pho81p, a cyclin-dependent kinase inhibitor in Saccharomyces cerevisiae. Knight, J.P., Daly, T.M., Bergman, L.W. Curr. Genet. (2004) [Pubmed]
  8. Promoter analysis of the PHO81 gene encoding a 134 kDa protein bearing ankyrin repeats in the phosphatase regulon of Saccharomyces cerevisiae. Ogawa, N., Noguchi, K., Yamashita, Y., Yasuhara, T., Hayashi, N., Yoshida, K., Oshima, Y. Mol. Gen. Genet. (1993) [Pubmed]
  9. Functional domains of Pho81p, an inhibitor of Pho85p protein kinase, in the transduction pathway of Pi signals in Saccharomyces cerevisiae. Ogawa, N., Noguchi, K., Sawai, H., Yamashita, Y., Yompakdee, C., Oshima, Y. Mol. Cell. Biol. (1995) [Pubmed]
  10. The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. Swinnen, E., Rosseels, J., Winderickx, J. Curr. Genet. (2005) [Pubmed]
  11. Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81. Lee, M., O'Regan, S., Moreau, J.L., Johnson, A.L., Johnston, L.H., Goding, C.R. Mol. Microbiol. (2000) [Pubmed]
  12. A putative membrane protein, Pho88p, involved in inorganic phosphate transport in Saccharomyces cerevisiae. Yompakdee, C., Ogawa, N., Harashima, S., Oshima, Y. Mol. Gen. Genet. (1996) [Pubmed]
  13. Function of the PHO regulatory genes for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. Yoshida, K., Ogawa, N., Oshima, Y. Mol. Gen. Genet. (1989) [Pubmed]
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