High impact information on APE2

• Both lap1 and lap4 were mapped to the left arm of chromosome XI, and lap3 was mapped to the left arm of chromosome XIV [1].
• In contrast, trimethionine, a model peptide used in transport assays, was not hydrolyzed by this aminopeptidase II-like activity, and the peptidase activity toward this substrate was localized in the soluble fraction of the yeast [2].
• APE2 is located on the X chromosome at position p11.21 and consists of six exons [3].
• APE2 was determined by DNA blot analysis to be a single-copy gene located on chromosome XI [4].
• The cloned APE2 gene was sequenced and found to consist of an open reading frame of 2583 bp encoding a protein of 861 amino acids [4].

Biological context of APE2

• Plasmids containing the gene encoding aminopeptidase yscII of Saccharomyces cerevisiae, APE2 (LAP1) were analyzed in detail [4].
• 2. From its substrate specificity and the effects of pH and inhibitors the enzyme was identified as aminopeptidase II [5].

Anatomical context of APE2

• Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipitation, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin [6].

Associations of APE2 with chemical compounds

• Plasmids capable of complementing lap1, lap2 and lap3 mutations [R.J. Trumbly and G. Bradley (1983) J. Bacteriol. 156, 36-48] were isolated from a yeast YEp13 library by screening for activity against the chromogenic aminopeptidase substrate L-leucine beta-naphthylamide in intact yeast colonies [4].

Other interactions of APE2

• Genetic mapping by tetrad analysis shows that the SPE1 gene is located on the left arm of chromosome XI, 6 cM from the LAP1 gene and 43 cM from the TRP3 gene [7].

Analytical, diagnostic and therapeutic context of APE2

• Yeast aminopeptidase II: rapid purification using affinity chromatography of the periplasmic enzyme [6].

References