High impact information on LOC85389

• In the spliceosomal C1 complex, snoRNP proteins efficiently assemble onto snoRNA sequences if they are located about 50 nt upstream of the intron branchpoint [1].
• Intron binding protein (IBP) 160, a helicase-like protein previously detected in the spliceosomal C1 complex, binds the pre-mRNA in a sequence-independent manner, contacting nucleotides 33-40 upstream of the intron branch site, regardless of whether a snoRNA is present [1].
• In the September 1 issue of Molecular Cell, demonstrate that a spliceosomal intron binding protein, IBP160, couples box C/D snoRNA processing with pre-mRNA splicing in the C1 splicing complex [2].
• Chromatin immunoprecipitation experiments show that polyadenylation factors and Rat1 localize to snoRNA genes, but mutations that disrupt poly(A) site cleavage or Rat1 activity do not lead to termination defects at these genes [3].
• The presence of a snoRNA inside an intron of the L1 ribosomal protein gene and the phylogenetic conservation of this gene arrangement suggest an important regulatory/functional link between these two components [4].

Biological context of LOC85389

• Mapping of ribosomal protein S3 and internally nested snoRNA U15A gene to human chromosome 11q13.3-q13.5 [5].
• These data suggest that trypanosomatids do not obey the general 5-bp rule for snoRNA-mediated methylation [6].
• Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene [7].
• In this study, a genome-wide analysis of the two major families of snoRNA genes in C. elegans was performed using the snoscan and snoGPS programs incorporating comparative genome analyses [8].
• snoSeeker: an advanced computational package for screening of guide and orphan snoRNA genes in the human genome [9].

Analytical, diagnostic and therapeutic context of LOC85389

• Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively [10].

References