Disease relevance of RPS3

• The N-terminal domain of the protein contains a highly negatively charged surface and resembles the fold of the N-terminal domain of Thermus thermophilus ribosomal protein S3 [1].
• Phylogenetic relationships among members of the class Mollicutes deduced from rps3 gene sequences [2].
• Additional confirmation of the phylogeny comes from the deduction that the UGA triplet encodes tryptophan in the rps3 gene from Mesoplasma florum, as it does in the mycoplasmas and spiroplasmas [2].
• Chromosomal instability of fanconi anemia cells is not the consequence of a defective repair activity of the ribosomal protein S3 [3].
• The human ribosomal protein S3 (rpS3) was expressed in E. coli using the pET-15b vector and the monoclonal antibodies (mAbs) were produced and characterized [4].

High impact information on RPS3

• A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3 [5].
• Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene [6].
• Therefore, we conclude that Hsp90 regulates the function of ribosomes by maintaining the stability of 40S ribosomal proteins such as rpS3 and rpS6 [7].
• Multifunctional ribosomal protein S3 (rpS3), a component of the ribosomal small subunit, is involved in DNA repair and apoptosis [7].
• Implication of mammalian ribosomal protein S3 in the processing of DNA damage [8].

Biological context of RPS3

• Mapping of ribosomal protein S3 and internally nested snoRNA U15A gene to human chromosome 11q13.3-q13.5 [9].
• Ribosomal protein S3 contains a potential nuclear localization signal, and the protein isolated as a nuclease also has a glycosylation pattern consistent with a nuclear localization as determined by lectin binding [8].
• Human ribosomal protein S3 interacts with DNA base excision repair proteins hAPE/Ref-1 and hOGG1 [10].
• In Xenopus S1 gene encoding ribosomal protein S3, however, three of its six introns contain U15 snoRNA gene sequence [11].
• In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in beta-cells [12].

Anatomical context of RPS3

• These results together indicated that all four genes, namely, rps3, rpl16, nad3 and rps12, were co-transcribed in rice mitochondria [13].
• Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin [14].
• Using the anti-rpS3 antibodies as probes, we investigated the cross-reactivities of various other mammalian brain tissues and cell lines, including human [4].
• Among the various regions in the brain tissues, the rpS3 positive neurons were predominantly observed in the ependymal cells, hippocampus and stantia nigra pars compacta [4].

Associations of RPS3 with chemical compounds

• Erk phosphorylates threonine 42 residue of ribosomal protein S3 [15].
• This sequence contains putative genes that encode initiator methionine tRNA (trnfM), subunits III (nad3) and IV (nad4) of the NADH dehydrogenase complex, and ribosomal proteins S3 (rps3), S12 (rps12) and L16 (rpl16) [16].

Other interactions of RPS3

• Moreover, the first intron of human RPS3 transcripts is processed to generate U15A, a small nucleolar RNA [9].
• Complete genomic structure of human rpS3: identification of functional U15b snoRNA in the fifth intron [11].
• During evolution the maize mitochondrial ribosomal protein subunit 12 (rps12) gene recombined with intron 1 of the ribosomal protein subunit 3 (rps3) gene and a region of the S1-like sequence of the 2.3 kb plasmid [17].
• The genes of the prokaryotic-like cistron that includes the rpsl9, rps3, rpl16, rpl5, and rpsl4 genes, which is at least partially conserved in the mitochondrial genomes of other higher and lower plants, are dispersed in the Oenothera mitochondrial genome [18].
• Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK [19].

Analytical, diagnostic and therapeutic context of RPS3

• A gene for a ribosomal protein, rps3, was amplified by PCR and sequenced from representatives of the class Mollicutes [2].
• In addition, sequence analysis of the clones also suggests that ribosomal protein S3 and cytochrome b as well as additional 4 uncharacterized genes may potentially be involved in P. aeruginosa pathogenesis [20].
• DNA analysis and Western blot analysis did not show significant differences in ribosomal protein S3 between FA cells and cells from healthy individuals [3].
• The binding affinity of mAbs to rpS3 determined by using a biosensor technology showed that they have similar binding affinities [4].
• Furthermore, these antibodies were successfully applied in immunohistochemistry in order to detect rpS3 in the gerbil brain tissues [4].

References