High impact information on DIS3

• Nuclear export of scR1 is mediated by the nuclear export signal receptor Xpo1p, is distinct from mRNA transport, and requires, as evidenced by the nucleolar accumulation of scR1 in a dis3/rrp44 exosome component mutant, an intact scR1 3' end [1].
• The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis [2].
• Here, we show that the cyt-4+ gene encodes a 120-kDa protein with significant similarity to the SSD1/SRK1 protein of Saccharomyces cerevisiae and the DIS3 protein of Schizosaccharomyces pombe, which have been implicated in protein phosphatase functions that regulate cell cycle and mitotic chromosome segregation [3].
• Increased dis3+ gene dosage reverses the Ts+ phenotype of a cdc25 wee1 strain, as does increased type 1 protein phosphatase gene dosage [4].
• The dis3+ gene is essential for growth; gene disruptant cells do not germinate and fail in cell division [4].

Biological context of DIS3

• Based on its nucleotide sequence, we cloned a full-sized human DIS3 cDNA [5].
• The predicted sts5+ gene product exhibits sequence similarity to two yeast proteins, Dis3 and Ssd1 and a nematode protein, F46E8.6, where the former two yeast proteins have been shown to be involved in cell cycle control and cell morphogenesis [6].
• The deduced amino acid sequences of these cDNAs revealed significant homology (45% identity) to the dis3+ gene product of Schizosaccharomyces pombe, a protein thought to be essential for mitotic control in the yeast [7].

Associations of DIS3 with chemical compounds

• ORF O2145 shows 41.2% identity with the glycophospholipid-anchored surface glycoprotein Gas1p of S. cerevisiae and ORF O2197 has 53.2% identity to chromosome segregation protein Dis3p of Schizosaccharomyces pombe [8].

Regulatory relationships of DIS3

• The SSD1 protein is implicated in G1 control and has a region of similarity to the dis3 protein of Schizosaccharomyces pombe [9].

Other interactions of DIS3

• In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3 [10].

References