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RPO31  -  DNA-directed RNA polymerase III core...

Saccharomyces cerevisiae S288c

Synonyms: DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit RPC1, O3254, RNA polymerase III subunit C1, RNA polymerase III subunit C160, ...
 
 
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Disease relevance of RPO31

  • The lengths of the mRNA that hybridized to phage lambda A-190 or lambda C-160 DNA on RNA blots were in agreement with the predicted sizes of the coding regions of the corresponding genes [1].
 

High impact information on RPO31

  • We have determined the nucleotide sequence of two yeast RNA polymerase genes, RPO21 and RPO31, which encode the largest subunits of RNA polymerases II and III, respectively [2].
  • The largest subunit of Pol III, C160, was identified as a target of Maf1 [3].
  • An extensive mutagenesis of domain f of C160, the largest subunit of RNA polymerase III, was carried out to better define its role and understand the mechanism through which C160 participates in transcription [4].
  • As predicted by our previous immunological results, yeast DNA inserts of the lambda A-190 and lambda C-160 clones cross-hybridized to the B-220 subunit gene [1].
  • We have used gel retardation and DNase protection assays to investigate the trans-acting factors involved in the regulation of yeast RNA polymerase genes RPC160 and RPC40 [5].
 

Biological context of RPO31

  • Upstream of the RPO31 gene, encoding the largest subunit of RNA polymerase III, lies a Abf1p binding site [6].
  • The N-terminal amino acid sequence of this polypeptide was shown to be identical to that predicted for a putative RPO31 polypeptide initiated at the first ATG codon located 208-216 base pairs 3' to the major RPO31 mRNA start sites [7].
  • A 18.4-kb fragment of the yeast genome containing the gene of the largest subunit of RNA polymerase C (RPC160) was cloned by hybridization to a previously isolated fragment of that gene [8].
  • RPC160 maps on chromosome XV, tightly linked but not allelic to the essential gene TSM8740 [8].
 

Other interactions of RPO31

  • Maf1p may play a role in the tRNA biosynthetic pathway since a fragment of the RPO31/RPC160 gene encoding the largest subunit of RNA polymerase III was cloned as a multicopy suppressor of mafl-1 [9].

References

  1. Isolation of structural genes for yeast RNA polymerases by immunological screening. Riva, M., Memet, S., Micouin, J.Y., Huet, J., Treich, I., Dassa, J., Young, R., Buhler, J.M., Sentenac, A., Fromageot, P. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  2. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Allison, L.A., Moyle, M., Shales, M., Ingles, C.J. Cell (1985) [Pubmed]
  3. General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1. Oficjalska-Pham, D., Harismendy, O., Smagowicz, W.J., Gonzalez de Peredo, A., Boguta, M., Sentenac, A., Lefebvre, O. Mol. Cell (2006) [Pubmed]
  4. Mutations in the alpha-amanitin conserved domain of the largest subunit of yeast RNA polymerase III affect pausing, RNA cleavage and transcriptional transitions. Thuillier, V., Brun, I., Sentenac, A., Werner, M. EMBO J. (1996) [Pubmed]
  5. ABF1 binding sites in yeast RNA polymerase genes. Della Seta, F., Treich, I., Buhler, J.M., Sentenac, A. J. Biol. Chem. (1990) [Pubmed]
  6. Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames. Wiemann, S., Rechmann, S., Benes, V., Voss, H., Schwager, C., Vlcek, C., Stegemann, J., Zimmermann, J., Erfle, H., Paces, V., Ansorge, W. Yeast (1996) [Pubmed]
  7. The RPO31 gene of Saccharomyces cerevisiae encodes the largest subunit of RNA polymerase III. Moyle, M., Hofmann, T., Ingles, C.J. Biochem. Cell Biol. (1986) [Pubmed]
  8. Conditional mutants of RPC160, the gene encoding the largest subunit of RNA polymerase C in Saccharomyces cerevisiae. Gudenus, R., Mariotte, S., Moenne, A., Ruet, A., Memet, S., Buhler, J.M., Sentenac, A., Thuriaux, P. Genetics (1988) [Pubmed]
  9. Mutation in a new gene MAF1 affects tRNA suppressor efficiency in Saccharomyces cerevisiae. Boguta, M., Czerska, K., Zoładek, T. Gene (1997) [Pubmed]
 
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