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Gene Review

RPC40  -  DNA-directed RNA polymerase core subunit...

Saccharomyces cerevisiae S288c

Synonyms: AC40, C37, C40, DNA-directed RNA polymerases I and III 40 kDa polypeptide, DNA-directed RNA polymerases I and III subunit RPAC1, ...
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Disease relevance of RPC40

  • However, the A135-AC40 interaction is weak compared with the E. coli alpha-beta interaction, and A135 mutation that abolishes the interaction is phenotypically silent [1].

High impact information on RPC40

  • The RPC40 gene is unique in the yeast genome and is required for cell viability [2].
  • Polyclonal antibodies outlined the alpha-like subunit AC40 and subunit AC19 which were found co-localized also in the apical region of the enzyme [3].
  • Four subunits that are common to pol I, II, and III (ABC27, ABC14.5, ABC10alpha, and ABC10beta), two that are common to pol I and III (AC40 and AC19), and one pol III-specific subunit (C11) can associate with defined regions of the two large subunits [4].
  • C53, a pol III-specific subunit, interacted with a 37-kDa polypeptide that copurifies with the enzyme and therefore appears to be a unique pol III subunit (C37) [4].
  • This gene is unique on the yeast genome and was located close to RPC40 and PRE2 on the right arm of chromosome XVI [5].

Biological context of RPC40

  • Furthermore, we have shown that rpa42+ complements a temperature-sensitive mutation in RPC40 the gene that encodes AC40 in S. cerevisiae and which is essential for cell growth [6].
  • Although the role of methylation of the wobble nucleotide is not known, the differences in elution properties from RPC-5 columns are consistent with other experimental observations indicating that a change in tRNA conformation accompanies this methylation [7].

Anatomical context of RPC40


Associations of RPC40 with chemical compounds

  • The human initiator tRNA made in yeast cells can be aminoacylated with methionine, and it was clearly separated from the yeast initiator and elongator methionine tRNAs by RPC-5 column chromatography [9].
  • RPC-5 contains dichlorodifluoroethylene beads coated with a quaternary ammonium compound where the substituents are: R1 = methyl, and R2-4 = C8-10 hydrocarbons [10].
  • The effect of zinc on the chromatographic behavior of four tRNAs was examined on RPC-5 and Aminex A-28 columns [10].

Other interactions of RPC40

  • Mutational analysis of the corresponding "alpha motif" indicated that its integrity is essential on AC40 subunit but is not essential on AC19 subunit [11].
  • Interactions between L and RPB3 or AC40 were, however, not detectable [12].
  • We have used gel retardation and DNase protection assays to investigate the trans-acting factors involved in the regulation of yeast RNA polymerase genes RPC160 and RPC40 [13].
  • We also demonstrate that determinants of RNAP assembly are conserved, and that a homologue of beta Asp(1084) in A135, the beta-like subunit of yeast RNAP I, is responsible for interaction with AC40, the largest alpha-like subunit [1].


  1. Inter- and intrasubunit interactions during the formation of RNA polymerase assembly intermediate. Naryshkina, T., Rogulja, D., Golub, L., Severinov, K. J. Biol. Chem. (2000) [Pubmed]
  2. RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C. Mann, C., Buhler, J.M., Treich, I., Sentenac, A. Cell (1987) [Pubmed]
  3. Localization of yeast RNA polymerase I core subunits by immunoelectron microscopy. Klinger, C., Huet, J., Song, D., Petersen, G., Riva, M., Bautz, E.K., Sentenac, A., Oudet, P., Schultz, P. EMBO J. (1996) [Pubmed]
  4. A protein-protein interaction map of yeast RNA polymerase III. Flores, A., Briand, J.F., Gadal, O., Andrau, J.C., Rubbi, L., Van Mullem, V., Boschiero, C., Goussot, M., Marck, C., Carles, C., Thuriaux, P., Sentenac, A., Werner, M. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  5. Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing. Hermann-Le Denmat, S., Werner, M., Sentenac, A., Thuriaux, P. Mol. Cell. Biol. (1994) [Pubmed]
  6. Isolation and characterization of the fission yeast gene rpa42+, which encodes a subunit shared by RNA polymerases I and III. Imazawa, Y., Imai, K., Fukushima, A., Hisatake, K., Muramatsu, M., Nogi, Y. Mol. Gen. Genet. (1999) [Pubmed]
  7. Dietary selenium affects methylation of the wobble nucleoside in the anticodon of selenocysteine tRNA([Ser]Sec). Diamond, A.M., Choi, I.S., Crain, P.F., Hashizume, T., Pomerantz, S.C., Cruz, R., Steer, C.J., Hill, K.E., Burk, R.F., McCloskey, J.A. J. Biol. Chem. (1993) [Pubmed]
  8. Sequence and codon recognition of bean mitochondria and chloroplast tRNAsTrp: evidence for a high degree of homology. Maréchal, L., Guillemaut, P., Grienenberger, J.M., Jeannin, G., Weil, J.H. Nucleic Acids Res. (1985) [Pubmed]
  9. Expression and function of a human initiator tRNA gene in the yeast Saccharomyces cerevisiae. Francis, M.A., Rajbhandary, U.L. Mol. Cell. Biol. (1990) [Pubmed]
  10. Effect of zinc ions on tRNA structure. I. Reversed-phase chromatography. Flanagan, J.M., Jacobson, K.B. J. Chromatogr. (1987) [Pubmed]
  11. Interactions between three common subunits of yeast RNA polymerases I and III. Lalo, D., Carles, C., Sentenac, A., Thuriaux, P. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  12. In vitro assembly of an archaeal D-L-N RNA polymerase subunit complex reveals a eukaryote-like structural arrangement. Eloranta, J.J., Kato, A., Teng, M.S., Weinzierl, R.O. Nucleic Acids Res. (1998) [Pubmed]
  13. ABF1 binding sites in yeast RNA polymerase genes. Della Seta, F., Treich, I., Buhler, J.M., Sentenac, A. J. Biol. Chem. (1990) [Pubmed]
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