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Gene Review

RPA190  -  DNA-directed RNA polymerase I core subunit...

Saccharomyces cerevisiae S288c

Synonyms: A190, DNA-directed RNA polymerase I 190 kDa polypeptide, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit RPA190, O6276, ...
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Disease relevance of RPA190


High impact information on RPA190

  • Furthermore, measurements of the exchange of A135 and A190 subunits between preexistent Pol I and newly synthesized Pol I showed that these two largest subunits of Pol I do not disassociate through many rounds of transcription in vivo [2].
  • As predicted by our previous immunological results, yeast DNA inserts of the lambda A-190 and lambda C-160 clones cross-hybridized to the B-220 subunit gene [3].
  • They all impair a two-hybrid interaction with a fragment conserved in the largest subunits of RNA polymerases I (Rpa190p), II (Rpb1p), and III (Rpc160p) [4].
  • In addition, the temperature-sensitive phenotype of the rpa12 null mutants can be partially suppressed by RPA190 (the gene for A190) on multicopy plasmids [5].
  • We have previously isolated mutants of Saccharomyces cerevisiae that are primarily defective in transcription of 35S rRNA genes by RNA polymerase I and have identified genes (RRN1 to RRN9) involved in this process [5].

Biological context of RPA190


Associations of RPA190 with chemical compounds

  • The synthesis of ribosomal proteins (r proteins) under the conditions of greatly reduced RNA synthesis were studied by using a strain of the yeast Saccharomyces cerevisiae in which the production of the largest subunit (RPA190) of RNA polymerase I was controlled by the galactose promoter [6].

Physical interactions of RPA190

  • In agreement with the fact that Rpb5 is shared among all three forms of eukaryotic RNA polymerases, the region H of RNA polymerase I subunit 1 (Rpa190) was also found to interact with Rpb5 [9].

Other interactions of RPA190

  • These results suggest that A12.2 plays a role in the assembly of A190 into a stable polymerase I structure [5].
  • A potential DNA-binding site (zinc-binding motif) is conserved in the N-terminal region I. Remarkably, the A190 subunit does not harbor the heptapeptide repeated sequence present in the B220 subunit [1].
  • RPA43 mapped next to RPA190, encoding the largest subunit of polymerase I. These genes are divergently transcribed and may thus share upstream regulatory elements ensuring their co-regulation [10].

Analytical, diagnostic and therapeutic context of RPA190

  • Separation of the protein subunit components by 5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by autoradiography and silver staining revealed that the two largest subunits (A190 and A135) were radiolabeled [11].


  1. RPA190, the gene coding for the largest subunit of yeast RNA polymerase A. Mémet, S., Gouy, M., Marck, C., Sentenac, A., Buhler, J.M. J. Biol. Chem. (1988) [Pubmed]
  2. RNA polymerase I remains intact without subunit exchange through multiple rounds of transcription in Saccharomyces cerevisiae. Schneider, D.A., Nomura, M. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  3. Isolation of structural genes for yeast RNA polymerases by immunological screening. Riva, M., Memet, S., Micouin, J.Y., Huet, J., Treich, I., Dassa, J., Young, R., Buhler, J.M., Sentenac, A., Fromageot, P. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  4. Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III. Briand, J.F., Navarro, F., Rematier, P., Boschiero, C., Labarre, S., Werner, M., Shpakovski, G.V., Thuriaux, P. Mol. Cell. Biol. (2001) [Pubmed]
  5. Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. Nogi, Y., Yano, R., Dodd, J., Carles, C., Nomura, M. Mol. Cell. Biol. (1993) [Pubmed]
  6. Conditional expression of RPA190, the gene encoding the largest subunit of yeast RNA polymerase I: effects of decreased rRNA synthesis on ribosomal protein synthesis. Wittekind, M., Kolb, J.M., Dodd, J., Yamagishi, M., Mémet, S., Buhler, J.M., Nomura, M. Mol. Cell. Biol. (1990) [Pubmed]
  7. Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Wittekind, M., Dodd, J., Vu, L., Kolb, J.M., Buhler, J.M., Sentenac, A., Nomura, M. Mol. Cell. Biol. (1988) [Pubmed]
  8. Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. McCusker, J.H., Yamagishi, M., Kolb, J.M., Nomura, M. Mol. Cell. Biol. (1991) [Pubmed]
  9. Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast. Miyao, T., Honda, A., Qu, Z., Ishihama, A. Mol. Gen. Genet. (1998) [Pubmed]
  10. Gene RPA43 in Saccharomyces cerevisiae encodes an essential subunit of RNA polymerase I. Thuriaux, P., Mariotte, S., Buhler, J.M., Sentenac, A., Vu, L., Lee, B.S., Nomura, M. J. Biol. Chem. (1995) [Pubmed]
  11. Yeast RNA polymerase I. Derivatization of the 190 and 135 subunits by 4-thiouridine monophosphate positioned uniquely at the 3' terminus of an enzyme-bound 32P-containing transcript initiated by a triribonucleotide primer on synthetic single-stranded DNA. Kelly, S., Sheng, N., Dennis, D. J. Biol. Chem. (1990) [Pubmed]
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