High impact information on ATG2

• We have identified and characterized a novel protein Apg2p essential for autophagy in yeast [1].
• Apg2p localizes to some membranous structure distinct from any known organelle [1].
• Apg2 localizes to the previously identified perivacuolar compartment that contains Apg9, the only characterized integral membrane protein that is required for autophagosome/Cvt vesicle formation [2].
• In this study, we have identified a novel protein, termed Apg2, required for both the Cvt and autophagy pathways as well as the specific degradation of peroxisomes [2].
• The majority of Cca1p-II and Cca1p-III, translated from ATG2 and ATG3, respectively, is in the cytosol but both are also located in the nucleus for processing precursors [3].

Biological context of ATG2

• Primer extension experiments show multiple transcription initiation sites upstream of ATG1 and between ATG1 and ATG2 [4].
• We here identify AUT8 as a novel gene essential for autophagy by screening a collection of approximately 5000 yeast deletion strains, each containing a defined deletion in an individual gene [5].
• Homozygous aut8 Delta cells are impaired in maturation of proaminopeptidase I, and they fail to undergo the cell differentiation process of sporulation [5].

Anatomical context of ATG2

• Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways [2].

Associations of ATG2 with chemical compounds

• The essential function of AUT8 for autophagy is further demonstrated by the lack of accumulation of autophagic vesicles in the vacuoles of aut8 Delta cells starved of nitrogen in the presence of the proteinase B inhibitor phenylmethylsulfonyl fluoride [5].

Analytical, diagnostic and therapeutic context of ATG2

• Some of the PCR products contained mutations in ATG1 and/or ATG2 [6].

References