High impact information on RPS3

• Overexpression of RPS3 suppresses both the stress sensitivity and the ribosome biogenesis defect of Deltayar1 mutants, but does not suppress either defect in Deltaltv1 mutants [1].
• Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae [1].
• The SUF14 gene is synonymous with RPS3, a single-copy essential gene that codes for the ribosomal protein S3 [2].
• The SUF13 and SUF14 genes were identified among extragenic suppressors of +1 frameshift mutations [2].
• A frameshift mutation in SUF14 that prevents full-length translation eliminated regulation, indicating that S3 is required for regulation [2].

Biological context of RPS3

• The promoter region of the RPS3 gene contains several upstream conserved sequence elements (UASrpg, T-rich region) that usually regulate transcription of ribosomal protein genes [3].
• The suppressors map at three loci, suf12, suf13, and suf14, which are located on chromosomes IV, XV, and XIV, respectively [4].

Analytical, diagnostic and therapeutic context of RPS3

• Electron microscopy indicates that pre-40S particles lack a structural landmark present in mature 40S subunits, the 'beak'. The beak is formed by the protrusion of 18S ribosomal RNA helix 33, which is in close vicinity to Rps3 [5].

References