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Gene Review

algB  -  two-component response regulator AlgB

Pseudomonas aeruginosa PAO1

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Disease relevance of algB

  • Identification of the histidine protein kinase KinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB [1].
  • When either purified E. coli IHF or extracts from P. aeruginosa were used in DNA binding studies, the algB mutant DNAs were severely reduced in IHF binding [2].

High impact information on algB

  • Two positive activators for alginate synthesis, algB and algR, are members of a superfamily of response regulators of the two-component regulatory system [1].
  • In this study, an open reading frame encoding a polypeptide of 66 kDa, designated kinB, was identified immediately downstream of algB [1].
  • We show that algB is transcriptionally active in mucoid strains of P. aeruginosa and regulates the expression of the alginate biosynthetic gene, algD, thereby resulting in increased expression of alginate in mucoid P. aeruginosa [3].
  • We determined the nucleotide sequence of the gene, algB, which regulates the level of exopolysaccharide produced by mucoid P. aeruginosa [3].
  • Of approximately 3,000 transposon mutants screened, 6 were found to display phenotypes which were mucoid relative to the phenotype of the parental algB strain [4].

Biological context of algB

  • A protein in cell extracts prepared from P. aeruginosa strains also demonstrated binding to algB fragments containing the IHF binding site, and the position of the complex formed with these extracts was identical to that of the complex formed with purified IHF [2].
  • The previously reported alg-50(Ts) mutation, which confers the phenotype of temperature-sensitive alginate production, was here designated as an algB allele [5].
  • An algB-cat gene fusion was constructed on a low-copy-number, broad-host-range plasmid [2].
  • The algB gene, which is involved in the production of alginate in Pseudomonas aeruginosa, was localized to approximately 2.2 kilobases of DNA from strain FRD by using transposon Tn501 insertion mutagenesis, subcloning, and complementation techniques [5].
  • In this study, transcription of algB, algD, and algR was characterized by gene fusion and primer extension analysis [6].

Anatomical context of algB

  • Mutations in algB, algR, and algT resulted in nonmucoid derivatives, yet algT mutants expressed flagella [7].

Associations of algB with chemical compounds

  • Recently, kinB was identified downstream of algB and was shown to encode the cognate histidine protein kinase that efficiently phosphorylates AlgB [8].

Analytical, diagnostic and therapeutic context of algB

  • In DNA gel band mobility shift assays, a protein present in extracts from IHF+ E. coli strains and IHF purified from E. coli bound specifically to these algB DNA fragments, while extracts prepared from isogenic IHF- E. coli strains failed to alter the mobility of algB DNA fragments containing the consensus IHF binding site [2].


  1. Identification of the histidine protein kinase KinB in Pseudomonas aeruginosa and its phosphorylation of the alginate regulator algB. Ma, S., Wozniak, D.J., Ohman, D.E. J. Biol. Chem. (1997) [Pubmed]
  2. Involvement of the alginate algT gene and integration host factor in the regulation of the Pseudomonas aeruginosa algB gene. Wozniak, D.J., Ohman, D.E. J. Bacteriol. (1993) [Pubmed]
  3. Pseudomonas aeruginosa AlgB, which modulates the expression of alginate, is a member of the NtrC subclass of prokaryotic regulators. Goldberg, J.B., Dahnke, T. Mol. Microbiol. (1992) [Pubmed]
  4. Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa. Woolwine, S.C., Wozniak, D.J. J. Bacteriol. (1999) [Pubmed]
  5. Construction and characterization of Pseudomonas aeruginosa algB mutants: role of algB in high-level production of alginate. Goldberg, J.B., Ohman, D.E. J. Bacteriol. (1987) [Pubmed]
  6. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB, and algD reveals a hierarchy of alginate gene expression which is modulated by algT. Wozniak, D.J., Ohman, D.E. J. Bacteriol. (1994) [Pubmed]
  7. Negative control of flagellum synthesis in Pseudomonas aeruginosa is modulated by the alternative sigma factor AlgT (AlgU). Garrett, E.S., Perlegas, D., Wozniak, D.J. J. Bacteriol. (1999) [Pubmed]
  8. Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa. Ma, S., Selvaraj, U., Ohman, D.E., Quarless, R., Hassett, D.J., Wozniak, D.J. J. Bacteriol. (1998) [Pubmed]
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