Disease relevance of amiR

• Nucleotide sequence of the aliphatic amidase regulator gene (amiR) of Pseudomonas aeruginosa [1].
• A plasmid, pCL34, capable of autonomous replication in Escherichia coli and Pseudomonas aeruginosa has been constructed which carries the promoter and structural gene (amiE) for P. aeruginosa amidase, but not the regulator gene (amiR) [2].

High impact information on amiR

• This promoter would be expected to provide basal levels of expression of the amiC and amiR regulatory genes to allow induction of the system [3].
• The nucleotide sequence of a 1001 bp ClaI/XhoI DNA fragment encoding the amidase regulator gene (amiR) from Pseudomonas aeruginosa has been determined [1].
• Analysis of the sequence has identified one of the reading frames as amiR [1].
• The amiR gene shows no sequence homology with other bacterial regulator proteins [1].
• A deletion within the presumed terminator region which disrupts the potential stem/loop formation leads to high constitutive amidase expression which is independent of the product of the regulator gene (amiR) [4].

Biological context of amiR

• Using plasmids with in vitro-constructed deletions, and plasmids containing subcloned DNA fragments, the amiR gene was located within a 1 kbp ClaI-XhoI DNA fragment [5].

References