High impact information on BAIAP3

• Using a two-hybrid system, we isolated a cDNA encoding a product that interacts with the cytoplasmic region of BAI1 and designated it BAP3 (BAI1-associated protein 3) [1].
• Cloning and characterization of BAP3 (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1 [1].
• The BAP3 product is a novel C2 domain-containing molecule with homology to Munc13 and synaptotagmin [1].
• One mAb, BAP-3, was found to react with all six individually expressed PSGs representing five alternatively spliced forms, but not with any of the seven expressed members of the carcinoembryonic antigen (CEA) subfamily [2].
• The BAP-3 epitope is located in the PSG B2 domain [2].

Biological context of BAIAP3

• BAIAP3 encodes a protein implicated in regulated exocytosis, which is colocalized with a secreted growth factor within cytoplasmic organelles [3].
• BAIAP3 therefore encodes a transcriptional target of an oncogenic fusion protein that implicates the regulated exocytotic pathway in cancer cell proliferation [3].
• Using the BAP-3 mAb, PSGs were found to be expressed exclusively by the syncytiotrophoblast of first trimester and term villi [2].

Associations of BAIAP3 with chemical compounds

• Deletion-mutant analysis revealed that the interaction between BAI1 and BAP3 was not mediated by the C2 domains [1].

Physical interactions of BAIAP3

• The BAIAP3 promoter is specifically bound in vivo by the (-KTS) isoform of EWS-WT1, consistent with its activation in reporter assays [3].

Other interactions of BAIAP3

• Induction of BAIAP3 by the EWS-WT1 chimeric fusion implicates regulated exocytosis in tumorigenesis [3].

References