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Gene Review

BTAF1  -  BTAF1 RNA polymerase II, B-TFIID...

Homo sapiens

Synonyms: ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, MOT1, TAF(II)170, TAF-172, ...
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Disease relevance of BTAF1


High impact information on BTAF1

  • Not only does TAF-172 activate pol III transcription, but it also inhibits the binding of TBP to the TATA box, thereby repressing pol II transcription [2].
  • We have previously shown that postnatal expression of the viral oncoprotein SV40 T antigen in rod photoreceptors (transgene MOT1), at a time when retinal cells have withdrawn from the mitotic cycle, leads to photoreceptor cell death (Al-Ubaidi et al., 1992. Proc. Natl. Acad. Sci. USA. 89:1194-1198) [3].
  • Taken together, these results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing [4].
  • This function is dependent on the presence of ATP in cell extracts but does not involve the ATPase activity of BTAF1 nor phosphorylation of NC2alpha [5].
  • Ribonuclease-protection experiments revealed that TAF(II)170 has multiple transcription start sites, consistent with the observation that the promoter lacks a canonical TATA box and initiator element [6].

Biological context of BTAF1

  • BTAF1 drastically changes the TATA-box binding specificity of TBP, as it is able to recruit DNA-binding defective TBP mutants to both TATA-containing and TATA-less DNA [7].
  • In this study we have isolated human genomic clones encoding TAF(II)170 and we show that the gene contains 37 introns [6].
  • 4. This locus, Mot1 (modifier of Tsc2 1), accounts for approximately 35% of the genetic variation in tumor size between the two strains [1].
  • Comparative genomics suggest that the Mot1 region is represented by human chromosomes 15q and 20pq [1].

Physical interactions of BTAF1

  • In addition, BTAF1 interacts with residues in helix 2 on the convex side of TBP as assayed in protein-protein and in DNA-binding assays [7].

Regulatory relationships of BTAF1

  • Furthermore, within this region the TAF(II)170 residues 290 to 381 can inhibit the interaction between Drosophila TAF(II)230 (residues 2 to 81) and TBP through competition for the concave surface of TBP [8].

Other interactions of BTAF1

  • Immunolabeling experiments localize the extreme carboxyl terminus of BTAF1 within the 170-kDa domain, whereas the amino terminus and TBP co-localize to the end of the protruding thumb [9].


  1. Genetic identification of a locus, Mot1, that affects renal tumor size in the rat. Yeung, R.S., Gu, H., Lee, M., Dundon, T.A. Genomics (2001) [Pubmed]
  2. The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB. Taggart, A.K., Fisher, T.S., Pugh, B.F. Cell (1992) [Pubmed]
  3. Bilateral retinal and brain tumors in transgenic mice expressing simian virus 40 large T antigen under control of the human interphotoreceptor retinoid-binding protein promoter. al-Ubaidi, M.R., Font, R.L., Quiambao, A.B., Keener, M.J., Liou, G.I., Overbeek, P.A., Baehr, W. J. Cell Biol. (1992) [Pubmed]
  4. Regulation of rRNA Synthesis by TATA-Binding Protein-Associated Factor Mot1. Dasgupta, A., Sprouse, R.O., French, S., Aprikian, P., Hontz, R., Juedes, S.A., Smith, J.S., Beyer, A.L., Auble, D.T. Mol. Cell. Biol. (2007) [Pubmed]
  5. NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein. Klejman, M.P., Pereira, L.A., van Zeeburg, H.J., Gilfillan, S., Meisterernst, M., Timmers, H.T. Mol. Cell. Biol. (2004) [Pubmed]
  6. The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization. Van Der Knaap, J.A., Van Den Boom, V., Kuipers, J., Van Eijk, M.J., Van Der Vliet, P.C., Timmers, H.T. Biochem. J. (2000) [Pubmed]
  7. Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants. Klejman, M.P., Zhao, X., van Schaik, F.M., Herr, W., Timmers, H.T. Nucleic Acids Res. (2005) [Pubmed]
  8. TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity. Pereira, L.A., van der Knaap, J.A., van den Boom, V., van den Heuvel, F.A., Timmers, H.T. Mol. Cell. Biol. (2001) [Pubmed]
  9. Molecular architecture of the basal transcription factor B-TFIID. Pereira, L.A., Klejman, M.P., Ruhlmann, C., Kavelaars, F., Oulad-Abdelghani, M., Timmers, H.T., Schultz, P. J. Biol. Chem. (2004) [Pubmed]
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