Disease relevance of cmk

• The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli [1].
• The Bacillus subtilis chromosome region encoding homologues of the Escherichia coli mssA and rpsA gene products [2].

High impact information on cmk

• Cloning and heterologous expression of Entamoeba histolytica adenylate kinase and uridylate/cytidylate kinase [3].
• In addition to the physiological studies, the region upstream of cmk was sequenced, and 120 codons with strong homology to an uncharacterized protein of the speB operon were identified [1].
• In a strain deleted for cmk, the pools of CMP and dCMP were elevated approximately 30-fold [1].
• We constructed a plasmid from which synthesis of CMP kinase was regulated by the lac promoter-operator and measured the synthesis rates for RNA and DNA after induction in the delta cmk/lacPO-cmk+ strain [1].
• Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli [4].

Biological context of cmk

• We have isolated and characterized two multicopy suppressors, mssA and mssB, which suppress the cold-sensitive growth phenotype of the smbA2 mutant of Escherichia coli [4].

Associations of cmk with chemical compounds

• Analysis of the conserved cmk rpsA cluster pinpointed the importance of cytosine nucleotide metabolism [5].

References