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Gene Review

rne  -  endoribonuclease; RNA-binding protein;RNA...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1069, JW1071, ams, hmp1, smbB
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Disease relevance of rne

  • Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12 [1].
  • A 16 kb transcript of the ams region, which is essential for biosynthesis of amylovoran, the acidic exopolysaccharide of Erwinia amylovora, was detected by Northern hybridization analysis [2].
  • The temperature-sensitive defects of both these strains are complemented by a recombinant lambda phage containing a genomic segment that carries the putative ams locus [3].

High impact information on rne

  • Highly purified RNAase E correctly processed E. coli 5S ribosomal RNA, bacteriophage T4 gene 32 mRNA and E. coli ompA mRNA at sites known to depend on the rne gene for cleavage in vivo [4].
  • The relationship between RNase K and the ams locus might point to a more general role of RNase K in mRNA degradation [5].
  • In vivo both activities are affected by a conditional mutation of the ams/rne gene that seems to be complemented at nonpermissive temperatures by a fragment of the groEL gene [6].
  • The product of the ams/rne/hmp1 gene, which is required for RNase E activity, was overexpressed, purified to near homogeneity by electroelution from an SDS/polyacrylamide gel, and renatured [7].
  • Here, we show that the half-lives of both the pnp and rne transcripts are dependent on the intracellular level of polyadenylated transcripts [8].

Biological context of rne

  • The translated product of the rne gene migrates in SDS containing polyacrylamide gels as a 110,000 Da polypeptide, but the open reading frame found in the sequenced DNA indicates a much smaller protein [9].
  • We have sequenced a 3.2 kb EcoRI-BamHI fragment encoding the rne gene, and identified its reading frame [9].
  • Low copy number or single copy number vectors carrying rne transcribed from either p1, p2 or p3 alone complement the rne 1018::bla deletion mutation at 30 degrees C, 37 degrees C and 44 degrees C. However, normal autoregulation requires the presence of all three promoters [10].
  • The results clearly indicated that the S. coelicolor RNase ES can partially abrogate either the rng::cat or rne-1 mutation, as measured by the ability to suppress the several aberrant phenotypes resulting from the rng or rne mutation [11].
  • The hmp1 gene is in fact identical to ams (abnormal mRNA stability), also designated rne (RnaseE), and reported to have an ORF of 91 kDa [12].

Anatomical context of rne

  • Analysis of interplay between translation mutations and rne function, together with the above observations, suggests that translation stabilizes messages in a general way against rne-dependent endonucleolytic cleavage, and that significant protection may be conferred by one or a few ribosomes [13].

Associations of rne with chemical compounds

  • The entity that migrates as a 110,000 Da contains RNA, which could account, at least partially, for the migration of the rne gene product in SDS containing polyacrylamide gels [9].


  1. Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12. Arraiano, C.M., Yancey, S.D., Kushner, S.R. J. Bacteriol. (1988) [Pubmed]
  2. Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Bugert, P., Geider, K. Mol. Microbiol. (1995) [Pubmed]
  3. Genetic studies of cleavage-initiated mRNA decay and processing of ribosomal 9S RNA show that the Escherichia coli ams and rne loci are the same. Melefors, O., von Gabain, A. Mol. Microbiol. (1991) [Pubmed]
  4. Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Carpousis, A.J., Van Houwe, G., Ehretsmann, C., Krisch, H.M. Cell (1994) [Pubmed]
  5. Cleavages in the 5' region of the ompA and bla mRNA control stability: studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease. Lundberg, U., von Gabain, A., Melefors, O. EMBO J. (1990) [Pubmed]
  6. Functional interaction of heat shock protein GroEL with an RNase E-like activity in Escherichia coli. Sohlberg, B., Lundberg, U., Hartl, F.U., von Gabain, A. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  7. RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product. Cormack, R.S., Genereaux, J.L., Mackie, G.A. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  8. Polyadenylation of Escherichia coli transcripts plays an integral role in regulating intracellular levels of polynucleotide phosphorylase and RNase E. Mohanty, B.K., Kushner, S.R. Mol. Microbiol. (2002) [Pubmed]
  9. Sequencing and expression of the rne gene of Escherichia coli. Chauhan, A.K., Miczak, A., Taraseviciene, L., Apirion, D. Nucleic Acids Res. (1991) [Pubmed]
  10. RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters. Ow, M.C., Liu, Q., Mohanty, B.K., Andrew, M.E., Maples, V.F., Kushner, S.R. Mol. Microbiol. (2002) [Pubmed]
  11. RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli. Inagawa, T., Okamoto, S., Wachi, M., Ochi, K. Biosci. Biotechnol. Biochem. (2003) [Pubmed]
  12. Cloning and analysis of the entire Escherichia coli ams gene. ams is identical to hmp1 and encodes a 114 kDa protein that migrates as a 180 kDa protein. Casarégola, S., Jacq, A., Laoudj, D., McGurk, G., Margarson, S., Tempête, M., Norris, V., Holland, I.B. J. Mol. Biol. (1992) [Pubmed]
  13. IS10 mRNA stability and steady state levels in Escherichia coli: indirect effects of translation and role of rne function. Jain, C., Kleckner, N. Mol. Microbiol. (1993) [Pubmed]
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