Disease relevance of katE

• Polyamine-deficient E. coli mutant has increased susceptibility to exogenous H2O2, and this cell cytotoxicity was relieved to a wild-type level by addition of putrescine or spermidine (1mM), which restored the transcriptional induction of ahpC, katG, and katE genes [1].
• We now report that the hypersensitivity of Deltafur mutants to UVA irradiation is associated with reduced hydroperoxidase I (HPI) and hydroperoxidase II (HPII) activity, and is associated with a decrease in the transcription of katE and katG genes [2].

High impact information on katE

• Forty-eight independent gene fusions were identified, including several in well-characterized RpoS-regulated genes, such as osmY, katE, and otsA [3].
• We isolated an extragenic mutation that affects the expression of the katE-lacZ fusion gene in the deltahns background [4].
• Amplification of portions of the intergenic spacer between the katE gene and cryptic cel operon of Escherichia coli was accomplished by the polymerase chain reaction using the DNA polymerase from Thermus aquaticus [5].
• A 3,466-bp nucleotide sequence containing the katE gene of Escherichia coli has been determined [6].
• Site-directed mutagenesis of the katE gene of Escherichia coli was used to change, individually and in combination, Cys438 and Cys669 to serine in catalase HPII [7].

Other interactions of katE

• In order to understand the molecular basis of selective expression of stationary-phase genes by RNA polymerase containing sigma38 (Esigma38) in Escherichia coli, we examined transcription from the stationary-phase promoters, katEP, bolAP, hdeABP, csgBAP, and mcbP, in vivo and in vitro [8].

References