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Gene Review

ruvB  -  ATP-dependent DNA helicase, component of...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1861, JW1849
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Disease relevance of ruvB

  • BACKGROUND: All the ruvA, ruvB and ruvC mutants of Escherichia coli are sensitive to treatments that damage DNA, and are mildly defective in homologous recombination [1].
  • We report here the cloning of the ruvB gene from a thermophilic eubacterium, Thermus thermophilus HB8 (Tth), and the biochemical characterization of the gene product expressed in E. coli [2].

High impact information on ruvB

  • The Escherichia coli ruvA and ruvB genes are involved in DNA repair and in the late step of homologous genetic recombination [3].
  • Molecular characterization of the locus revealed that there are three genes--ruvA, ruvB and ruvC; mutations in any one of the genes confer the same phenotype [4].
  • K68R, K68A, and T69A mutants except T69S failed to complement UV-sensitive phenotype of the ruvB strain [5].
  • To elucidate the role of the Walker motif A of RuvB (GXGKT; X indicates a nonconserved residue) in ATP hydrolysis and branch migration activities, we constructed four ruvB mutant genes by site-directed mutagenesis, altering the highly conserved Lys(68) and Thr(69) [5].
  • In this study, we isolated a large number of plasmids carrying ruvB mutant genes and identified amino acid residues important for the RuvB functions by examining the in vivo DNA repair activities of the mutant proteins [6].

Biological context of ruvB


Other interactions of ruvB

  • B. burgdorferi ruvB is part of an operon which comprises the homologues of ruvA, queA and pfbB [11].
  • Expression of the B. burgdorferi ruvB and ruvA genes renders a wild-type Escherichia coli sensitive to UV light and mitomycin, indicative of negative complementation. priA, which encodes the potential recognition factor for the primosome assembly site, was found at 15 kbp from the left telomere [11].

Analytical, diagnostic and therapeutic context of ruvB

  • To gain insight into the mechanism of branch migration, random mutations were introduced into the ruvB gene by PCR and a collection of mutant alleles were obtained [12].


  1. Abortive recombination in Escherichia coli ruv mutants blocks chromosome partitioning. Ishioka, K., Fukuoh, A., Iwasaki, H., Nakata, A., Shinagawa, H. Genes Cells (1998) [Pubmed]
  2. Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions. Yamada, K., Fukuoh, A., Iwasaki, H., Shinagawa, H. Mol. Gen. Genet. (1999) [Pubmed]
  3. Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Iwasaki, H., Takahagi, M., Nakata, A., Shinagawa, H. Genes Dev. (1992) [Pubmed]
  4. RuvA, RuvB and RuvC proteins: cleaning-up after recombinational repairs in E. coli. Kuzminov, A. Bioessays (1993) [Pubmed]
  5. Role of walker motif A of RuvB protein in promoting branch migration of holliday junctions. Walker motif a mutations affect Atp binding, Atp hydrolyzing, and DNA binding activities of Ruvb. Hishida, T., Iwasaki, H., Yagi, T., Shinagawa, H. J. Biol. Chem. (1999) [Pubmed]
  6. Mutational analysis of the functional motifs of RuvB, an AAA+ class helicase and motor protein for holliday junction branch migration. Iwasaki, H., Han, Y.W., Okamoto, T., Ohnishi, T., Yoshikawa, M., Yamada, K., Toh, H., Daiyasu, H., Ogura, T., Shinagawa, H. Mol. Microbiol. (2000) [Pubmed]
  7. Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes. Benson, F.E., Illing, G.T., Sharples, G.J., Lloyd, R.G. Nucleic Acids Res. (1988) [Pubmed]
  8. Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion. Mendonca, V.M., Matson, S.W. Genetics (1995) [Pubmed]
  9. RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro. Tsaneva, I.R., Müller, B., West, S.C. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  10. Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. Iwasaki, H., Shiba, T., Makino, K., Nakata, A., Shinagawa, H. J. Bacteriol. (1989) [Pubmed]
  11. Homologues of helicase genes priA and ruvAB of Borrelia burgdorferi, the Lyme borreliosis agent. Boursaux-Eude, C., Margarita, D., Belfaiza, J., Old, I.G., Saints Girons, I. Res. Microbiol. (1998) [Pubmed]
  12. Escherichia coli RuvBL268S: a mutant RuvB protein that exhibits wild-type activities in vitro but confers a UV-sensitive ruv phenotype in vivo. Mézard, C., George, H., Davies, A.A., van Gool, A.J., Zerbib, D., Stasiak, A., West, S.C. Nucleic Acids Res. (1999) [Pubmed]
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