Disease relevance of cspE

• Screening for mutations among common laboratory E. coli strains showed a high degree of genetic diversity in cspC but not in cspA and cspE [1].

High impact information on cspE

• Characterization of Escherichia coli cspE, whose product negatively regulates transcription of cspA, the gene for the major cold shock protein [2].
• CspE is highly produced at 37 degrees C. The synthesis level of CspE transiently increased during the growth lag period after dilution of stationary-phase cells into the fresh medium at 37 degrees C. This is consistent with the delta cspE phenotype of the longer growth lag period after dilution [2].
• DNA sequencing revealed that the msmB gene was identical to the cspC gene and that the msmC gene had not been described before [3].
• Deletion of crcA, cspE and crcB exacerbates the nucleoid morphology defects of the topo IV Ts mutants [4].
• These activities were augmented when crcB was overexpressed with cspE (100-fold camphor resistance and 2.1-fold induction of rcsA) [4].

Chemical compound and disease context of cspE

• The authors have previously shown that overexpression of the Escherichia coli K-12 crcA, cspE and crcB genes protects the chromosome from decondensation by camphor [4].
• When present on a multicopy plasmid, gicA conferred UV sensitivity on those strains of E. coli K-12, in which it produces the cold-dependent growth retardation and streptomycin sensitivity but did not confer any gamma radiation sensitivity [5].

Biological context of cspE

• In this study, we analyzed global transcript profiles of cells lacking cspE and cspC as well as cells individually overexpressing these proteins or a CspE mutant that is unable to melt nucleic acids and is defective in cold acclimation [6].

Other interactions of cspE

• In the delta cspE strain, the synthesis of a number of proteins at 37 degrees C was found to be altered and cspA was derepressed [2].

Analytical, diagnostic and therapeutic context of cspE

• The protein synthesis patterns of the delta cspE strain and the wild-type strain were compared using two-dimensional gel electrophoresis [2].

References