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Gene Review:

rpsD - 30S ribosomal subunit protein S4

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3283, JW3258, ramA, sud

Grundy, F.J. et al., Grundy, F.J. et al., Komeda, H. et al., Grundy, F.J. et al., van der Straaten, T. et al.

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Disease relevance of rpsD

The leader region of rpsD mRNA is predicted to have extensive secondary structure, resembling a region of B. subtilis 16S rRNA where S4 is likely to bind; similar mRNA features have been found to be important in ribosomal gene regulation in E. coli [1].
The rpsD gene, encoding ribosomal protein S4, was isolated from Bacillus subtilis by hybridization with oligonucleotide probes derived from the S4 amino-terminal protein sequence [1].
Salmonella gene rma (ramA) and multiple-drug-resistant Salmonella enterica serovar typhimurium [2].
The amount of R-amidase in the supernatant of the sonicated cell-free extract of an Escherichia coli transformant overexpressing the ramA gene was about 30 000 times higher than that of Pseudomonas sp. MCI3434 [3].

High impact information on rpsD

The first of these helices has a side loop which is essential for autoregulation, is highly conserved among Bacillus rpsD genes, and is similar to a region of 16S rRNA important in S4 binding [4].
In E. coli, rpsD is located in the alpha operon, and S4 acts as the translational regulator for alpha operon expression, binding to a target site in the alpha operon mRNA [5].
An open reading frame homologous to tyrosyl-tRNA synthetase (tyrS) genes was identified downstream of rpsD but in the opposite orientation [1].
Sequence analysis of the cloned DNA indicated that rpsD is likely to be monocistronic, in contrast to Escherichia coli rpsD, which is located in the alpha operon and is the translational regulator for alpha operon ribosomal protein gene expression in E. coli [1].
Analysis of 1332 bp of the genomic DNA revealed the presence of one open reading frame (ramA) which encodes the R-amidase [3].

Biological context of rpsD

These results provide the first steps toward analysis of the regulation of rpsD gene expression in B. subtilis [1].

Analytical, diagnostic and therapeutic context of rpsD

The target site for repression of B. subtilis rpsD by protein S4 was localized by deletion and oligonucleotide-directed mutagenesis to the leader region of the monocistronic rpsD gene [5].

References

Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4. Grundy, F.J., Henkin, T.M. J. Bacteriol. (1990) [Pubmed]
Salmonella gene rma (ramA) and multiple-drug-resistant Salmonella enterica serovar typhimurium. van der Straaten, T., Janssen, R., Mevius, D.J., van Dissel, J.T. Antimicrob. Agents Chemother. (2004) [Pubmed]
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide. Komeda, H., Harada, H., Washika, S., Sakamoto, T., Ueda, M., Asano, Y. Eur. J. Biochem. (2004) [Pubmed]
Characterization of the Bacillus subtilis rpsD regulatory target site. Grundy, F.J., Henkin, T.M. J. Bacteriol. (1992) [Pubmed]
The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis. Grundy, F.J., Henkin, T.M. J. Bacteriol. (1991) [Pubmed]

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