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Gene Review

dnapol  -  ORF114

Mamestra configurata nucleopolyhedrovirus B

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Disease relevance of dnapol

  • The polyhedrin gene is located from 0.0 to 2.0 m.u. and two other genes, dnapol and p143, both of which are essential for baculovirus DNA replication, are located from 35.3 to 40.9 m.u. and 55.5 to 63.4 m.u., respectively [1].
  • While less protein was obtained from the E. coli expression system, SpliNPV DNAPOL purified from E. coli displayed similar biochemical characteristics to DNAPOL expressed in, and subsequently purified from, insect cells (Sf9) using a baculovirus expression system [2].

High impact information on dnapol

  • Coexpression with ie0 and/or ie1 immediate-early genes dramatically enhanced dnapol transcription, specifically from a new start site [3].
  • The dnapol promoter region contains a putative overlapping open reading frame (ORF) in the opposite direction [3].
  • We showed that ORF-2 was indeed highly expressed late, when the dnapol mRNA level decreased, and that during that time, dnapol mRNA stability was not significantly altered, excluding a destabilizing antisense effect [3].
  • Finally, we showed that both IE1 transactivation and late inhibition occurred in the same limited region around the dnapol promoter [3].
  • To reconcile the conflicting data and determine if the AcMNPV DNA polymerase is required for viral DNA replication during the course of an infection, a dnapol-null virus was generated using bacmid technology [4].

Biological context of dnapol


Analytical, diagnostic and therapeutic context of dnapol


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