Disease relevance of HzsnnVgp002

• The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity [1].
• Experiments with the unique orf2 bro gene of Autographa californica multicapsid nucleopolyhedrovirus showed that it is not required for replication, but may enhance replication during the occlusion phase of reproduction [2].
• We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus [1].
• Immunoelectron microscopic analyses indicated that ORF2 was associated with the ends of the capsids which contain the basal structure [3].

High impact information on HzsnnVgp002

• Transcriptional mapping indicated the ORF2 transcripts were initiated at a late promoter sequence, ATAAG, beginning about 24 hr p.i. These transcripts terminated near the 3' end of the ORF2 reading frame [3].
• Antibodies were produced against a fusion protein derived from the bacterial gene encoding the maltose binding protein and most of the ORF2 sequence [3].
• Sequence analysis revealed the presence of point mutations in ORF2 and in lef-2 [4].
• Sequencing of the dnapol promoter region of Autographa californica nuclear polyhedrosis virus (AcNPV) revealed an overlapping open reading frame (ORF) in an antisense orientation, referred to as ORF-2 [5].
• Analysis of the ORF-2 deduced amino-acid sequence revealed two short regions of homology with a similar ORF from Lymantria dispar nuclear polyhedrosis virus (LdNPV) [5].

Biological context of HzsnnVgp002

• The 5' regulatory region of ORF2 which can encode a protein with 115 amino acid residues contains only an early transcriptional initiation motif [6].

Associations of HzsnnVgp002 with chemical compounds

• Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis [1].

Analytical, diagnostic and therapeutic context of HzsnnVgp002

• We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus [1].
• A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5' regulatory elements of baculovirus early genes [7].

References