Disease relevance of Methanosarcinaceae

• Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli [1].
• The R. rubrum CODH is 67% similar to the beta subunit of the Clostridium thermoaceticum CODH and 47% similar to the alpha subunit of the Methanothrix soehngenii CODH; an alignment of these three peptides shows relatively limited overall conservation [2].
• In contrast with the carbon monoxide dehydrogenases from most other anaerobic bacteria, the purified enzyme of Methanothrix soehngenii was remarkably stable towards oxygen and it was only slightly inhibited by cyanide [3].

High impact information on Methanosarcinaceae

• Pyrrolysine is an amino acid encoded by the amber codon in genes required for methylamine utilization by members of the Methanosarcinaceae [4].
• Cloning, expression, and sequence analysis of the genes for carbon monoxide dehydrogenase of Methanothrix soehngenii [5].
• The chromosomal protein MC1 of Methanothrix soehngenii is a family of three variants a, b, and c. These are small basic polypeptides of 89, 87, and 90 residues, respectively [6].
• Low temperature regulated DEAD-box RNA helicase from the Antarctic archaeon, Methanococcoides burtonii [7].
• Structures of minor ether lipids isolated from the aceticlastic methanogen, Methanothrix concilii GP6 [8].

Biological context of Methanosarcinaceae

• The extent of sequence homologies between protein MC1 from M. soehngenii and proteins MC1 from two other species of Methanosarcinaceae is only 60% [6].

Associations of Methanosarcinaceae with chemical compounds

• The mcrA clone libraries also included phylotypes related to the methyl-disproportionating genus Methanococcoides [9].
• Isolation and characterization of acetyl-coenzyme A synthetase from Methanothrix soehngenii [10].
• Partial gene sequences for the A subunit of methyl-coenzyme M reductase (mcrI) as a phylogenetic tool for the family Methanosarcinaceae [11].
• Acetate and CO2 assimilation by Methanothrix concilii [12].
• Propionate-grown granular sludge consisted of two types of clusters, those of a rod-shaped bacterium immunologically related to Methanothrix soehngenii and those consisting of two different types of bacteria with a specific spatial orientation [13].

Gene context of Methanosarcinaceae

• This protein immunoreacted with antiserum raised against purified Acs isolated from an unrelated species, Methanothrix soehngenii [14].
• Due to their presumed importance in removing cold-stabilised secondary structures in mRNA, we have characterised a putative DEAD-box RNA helicase gene (deaD) from the Antarctic methanogen, Methanococcoides burtonii [7].
• Membrane ATPase from the aceticlastic methanogen Methanothrix thermophila [15].
• The kinetic properties of the acetyl-CoA synthetase can explain the high affinity for acetate of Methanothrix soehngenii [10].
• In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs) [1].

References